Difference between revisions of "LsrA/cI"
From GcatWiki
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|'''Step'''|| '''BBa_''' || '''Description''' ||''' Prefix/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Prefix/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size''' | |'''Step'''|| '''BBa_''' || '''Description''' ||''' Prefix/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Prefix/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size''' | ||
|- | |- | ||
| − | | 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| || | + | | 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||132 |
|- | |- | ||
| − | | 2|| ||pBAD+RBS ||R/X; S/P ||S/P ||132 ||K091106 ||lsrR ||R/X;S/P ||X/P ||954 || || | + | | 2|| ||pBAD+RBS ||R/X; S/P ||S/P ||132 ||K091106 ||lsrR ||R/X;S/P ||X/P ||954 || ||1068 |
|- | |- | ||
|3 ||C0051 || cI ||R/X;S/P ||S/P ||711 ||B0015 ||double terminator ||R/X;S/P ||X/P ||129 || || | |3 ||C0051 || cI ||R/X;S/P ||S/P ||711 ||B0015 ||double terminator ||R/X;S/P ||X/P ||129 || || | ||
Revision as of 14:13, 2 July 2008
Goal: pBAD+RBS+lsrR+TT+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT
I13453+B0034+C0079+B0015+C0051+B0015+K091106+B0034+B0034+C0072+B0015
| Vector | Insert | Product | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Step | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Size |
| 1 | I13453 | pBAD | R/X;S/P | S/P | 120 | B0034 | RBS | R/X; S/P | X/P | 12 | 132 | |
| 2 | pBAD+RBS | R/X; S/P | S/P | 132 | K091106 | lsrR | R/X;S/P | X/P | 954 | 1068 | ||
| 3 | C0051 | cI | R/X;S/P | S/P | 711 | B0015 | double terminator | R/X;S/P | X/P | 129 | ||
