Difference between revisions of "LsrA/cI"
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Revision as of 14:42, 2 July 2008
Goal: pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015
Vector | Insert | Product | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Step | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Size |
1 | I13453 | pBAD | R/X;S/P | S/P | 120 | B0034 | RBS | R/X; S/P | X/P | 12 | ||
2 | K091106 | lsrR | R/X; S/P | R/X | 954 | pBAD+RBS | R/X;S/P | R/S | ||||
3 | pBAD+RBS+lsrR | R/X; S/P | S/P | P0455 | RBS+cI+TT | R/X; S/P | X/P | 896 | ||||