Difference between revisions of "LsrA/cI"
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| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || || | | 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || || | ||
|- | |- | ||
| − | | 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || || || | + | | 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| || |
|- | |- | ||
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || || | | 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || || | ||
Revision as of 14:50, 2 July 2008
Goal: pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015
| Vector | Insert | Product | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Step | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Size |
| 1 | I13453 | pBAD | R/X;S/P | S/P | 120 | B0034 | RBS | R/X; S/P | X/P | 12 | ||
| 2 | K091001 | lsrR | R/X; S/P | R/X | 954 | pBAD+RBS | R/X;S/P | R/S | ||||
| 3 | pBAD+RBS+lsrR | R/X; S/P | S/P | P0455 | RBS+cI+TT | R/X; S/P | X/P | 896 | ||||
| 4 | C0261 | RBS+luxI | R/X; S/P | R/X | 661 | K091106 | pLsrA/cI | R/X; S/P | R/S | 141 | ||
| 5 | I13025 | RBS+Mnt | R/X;S/P | S/P | 331 | B0015 | TT | R/X; S/P | X/P | 129 | ||
| 6 | ||||||||||||
| 7 |
