Difference between revisions of "LsrA/cI"

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I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015
 
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015
  
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'''Ligation Plan'''
 
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| 7|| ||pBAD+RBS+lsrR+RBS+cI+TT  ||R/X; S/P  ||S/P  || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || ||
 
| 7|| ||pBAD+RBS+lsrR+RBS+cI+TT  ||R/X; S/P  ||S/P  || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || ||
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'''Parts'''
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!width="100"|Description
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!width="30"|Source
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!width="30"|Responsible
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!width="60"|location(07/08)
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!width="30"|Vector
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Revision as of 15:03, 2 July 2008

Goal: pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT

I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015


Ligation Plan

Vector Insert Product
Step BBa_ Description Pre-/suffix Enzymes Size BBa_ Description Pre-/suffix Enzymes Size BBa_ Size
1 I13453 pBAD R/X;S/P S/P 120 B0034 RBS R/X; S/P X/P 12
2 K091001 lsrR R/X; S/P R/X 954 pBAD+RBS R/X;S/P R/S
3 pBAD+RBS+lsrR R/X; S/P S/P P0455 RBS+cI+TT R/X; S/P X/P 896
4 C0261 RBS+luxI R/X; S/P R/X 661 K091106 pLsrA/cI R/X; S/P R/S 141
5 I13025 RBS+Mnt R/X;S/P S/P 331 B0015 TT R/X; S/P X/P 129
6 pLsrA/cI+RBS+luxI R/X; S/P S/P RBS+Mnt+TT R/X; S/P X/P
7 pBAD+RBS+lsrR+RBS+cI+TT R/X; S/P S/P pLsrA/cI+RBS+luxI+RBS+Mnt+TT R/X; S/P X/P

Parts

BBa_ Description Source Responsible location(07/08) Vector
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