Difference between revisions of "LsrA/cI"

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|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07  ||Plt.1-16M ||pSB1AC3 ||  
 
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07  ||Plt.1-16M ||pSB1AC3 ||  
 
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−
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+
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW  ||N/A||N/A ||
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 +
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW  ||N/A ||N/A ||  
 
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Revision as of 15:24, 2 July 2008

Goal: pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT

I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015


Ligation Plan

Vector Insert Product
Step BBa_ Description Pre-/suffix Enzymes Size BBa_ Description Pre-/suffix Enzymes Size BBa_ Size
1 I13453 pBAD R/X;S/P S/P 120 B0034 RBS R/X; S/P X/P 12
2 K091001 lsrR R/X; S/P R/X 954 pBAD+RBS R/X;S/P R/S
3 pBAD+RBS+lsrR R/X; S/P S/P P0455 RBS+cI+TT R/X; S/P X/P 896
4 C0261 RBS+luxI R/X; S/P R/X 661 K091106 pLsrA/cI R/X; S/P R/S 141
5 I13025 RBS+Mnt R/X;S/P S/P 331 B0015 TT R/X; S/P X/P 129
6 pLsrA/cI+RBS+luxI R/X; S/P S/P RBS+Mnt+TT R/X; S/P X/P
7 pBAD+RBS+lsrR+RBS+cI+TT R/X; S/P S/P pLsrA/cI+RBS+luxI+RBS+Mnt+TT R/X; S/P X/P

Parts

BBa_ Description Source location('07) Vector Responsible
B0015 double terminator registry 07 Plt.1-1I;Plt.3-3O pSB1AK3
B0034 RBS registry 07 Plt.1-3O pSB1A2
I13453 pBAD registry 07 Plt.2-13D; Plt.3-3I pSB1A3
C0261 RBS+luxI registry 07 Plt.1-24A pSB1A2
P0455 RBS+cI+TT registry 07 Plt.1-16M pSB1AC3
I13025 RBS+Mnt registry 07 Plt.1-23J pSB1A2
K091001 lsrR MW N/A N/A
K091106 plsrA/cI MW N/A N/A
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