Difference between revisions of "LsrA/cI"
From GcatWiki
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'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT | '''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT | ||
| − | I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015 | + | I13453+B0034+K091001+P0455(B0034+C0051+B0015)+K091106+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015 |
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!width="40"|Responsible | !width="40"|Responsible | ||
|- | |- | ||
| − | | [http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || | + | |[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || |
|- | |- | ||
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || | |[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || | ||
Revision as of 15:26, 2 July 2008
Goal: pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT
I13453+B0034+K091001+P0455(B0034+C0051+B0015)+K091106+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015
Ligation Plan
| Vector | Insert | Product | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Step | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Size |
| 1 | I13453 | pBAD | R/X;S/P | S/P | 120 | B0034 | RBS | R/X; S/P | X/P | 12 | ||
| 2 | K091001 | lsrR | R/X; S/P | R/X | 954 | pBAD+RBS | R/X;S/P | R/S | ||||
| 3 | pBAD+RBS+lsrR | R/X; S/P | S/P | P0455 | RBS+cI+TT | R/X; S/P | X/P | 896 | ||||
| 4 | C0261 | RBS+luxI | R/X; S/P | R/X | 661 | K091106 | pLsrA/cI | R/X; S/P | R/S | 141 | ||
| 5 | I13025 | RBS+Mnt | R/X;S/P | S/P | 331 | B0015 | TT | R/X; S/P | X/P | 129 | ||
| 6 | pLsrA/cI+RBS+luxI | R/X; S/P | S/P | RBS+Mnt+TT | R/X; S/P | X/P | ||||||
| 7 | pBAD+RBS+lsrR+RBS+cI+TT | R/X; S/P | S/P | pLsrA/cI+RBS+luxI+RBS+Mnt+TT | R/X; S/P | X/P |
Parts
| BBa_ | Description | Source | location('07) | Vector | Responsible |
|---|---|---|---|---|---|
| B0015 | double terminator | registry 07 | Plt.1-1I;Plt.3-3O | pSB1AK3 | |
| B0034 | RBS | registry 07 | Plt.1-3O | pSB1A2 | |
| I13453 | pBAD | registry 07 | Plt.2-13D; Plt.3-3I | pSB1A3 | |
| C0261 | RBS+luxI | registry 07 | Plt.1-24A | pSB1A2 | |
| P0455 | RBS+cI+TT | registry 07 | Plt.1-16M | pSB1AC3 | |
| I13025 | RBS+Mnt | registry 07 | Plt.1-23J | pSB1A2 | |
| K091001 | lsrR | MW | N/A | N/A | |
| K091106 | plsrA/cI | MW | N/A | N/A |
