Difference between revisions of "LsrA/cI"
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|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size''' | |'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size''' | ||
|- | |- | ||
| − | | 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| || | + | | 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12||TBD || |
|- | |- | ||
| − | | 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || || | + | | 2||K091001||lsrR ||R/X; S/P ||R/X ||954 ||TBD ||pBAD+RBS ||R/X;S/P ||R/S || || TBD|| |
|- | |- | ||
| − | | 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || || | + | | 3||TBD ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 ||TBD || |
|- | |- | ||
| − | | 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| || | + | | 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141||TBD|| |
|- | |- | ||
| − | | 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || || | + | | 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 ||TBD || |
|- | |- | ||
| − | | 6|| ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || || ||RBS+Mnt+TT ||R/X; S/P ||X/P || || || | + | | 6||TBD ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || ||TBD ||RBS+Mnt+TT ||R/X; S/P ||X/P || || TBD|| |
|- | |- | ||
| − | | 7|| ||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||S/P || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || || | + | | 7|| TBD||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||S/P || ||TBD ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || ||TBD || |
|- | |- | ||
|} | |} | ||
Revision as of 15:46, 2 July 2008
Goal:
pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT
I13453+B0034+K091001+P0455(B0034+C0051+B0015)+K091106+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015
Ligation Plan
| Vector | Insert | Product | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Step | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Size |
| 1 | I13453 | pBAD | R/X;S/P | S/P | 120 | B0034 | RBS | R/X; S/P | X/P | 12 | TBD | |
| 2 | K091001 | lsrR | R/X; S/P | R/X | 954 | TBD | pBAD+RBS | R/X;S/P | R/S | TBD | ||
| 3 | TBD | pBAD+RBS+lsrR | R/X; S/P | S/P | P0455 | RBS+cI+TT | R/X; S/P | X/P | 896 | TBD | ||
| 4 | C0261 | RBS+luxI | R/X; S/P | R/X | 661 | K091106 | pLsrA/cI | R/X; S/P | R/S | 141 | TBD | |
| 5 | I13025 | RBS+Mnt | R/X;S/P | S/P | 331 | B0015 | TT | R/X; S/P | X/P | 129 | TBD | |
| 6 | TBD | pLsrA/cI+RBS+luxI | R/X; S/P | S/P | TBD | RBS+Mnt+TT | R/X; S/P | X/P | TBD | |||
| 7 | TBD | pBAD+RBS+lsrR+RBS+cI+TT | R/X; S/P | S/P | TBD | pLsrA/cI+RBS+luxI+RBS+Mnt+TT | R/X; S/P | X/P | TBD |
Parts
| BBa_ | Description | Source | location('07) | Vector | Responsible |
|---|---|---|---|---|---|
| B0015 | double terminator | registry 07 | Plt.1-1I;Plt.3-3O | pSB1AK3 | |
| B0034 | RBS | registry 07 | Plt.1-3O | pSB1A2 | |
| I13453 | pBAD | registry 07 | Plt.2-13D; Plt.3-3I | pSB1A3 | |
| C0261 | RBS+luxI | registry 07 | Plt.1-24A | pSB1A2 | |
| P0455 | RBS+cI+TT | registry 07 | Plt.1-16M | pSB1AC3 | |
| I13025 | RBS+Mnt | registry 07 | Plt.1-23J | pSB1A2 | |
| K091001 | lsrR | MW | N/A | N/A | |
| K091106 | plsrA/cI | MW | N/A | N/A |
