Difference between revisions of "LsrA/cI"
From GcatWiki
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|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size''' | |'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size''' | ||
|- | |- | ||
− | | 1|| | + | | 1|| B0034 ||RBS ||R/X; S/P||S/P || 12||I13453||pBAD ||R/X;S/P ||X/P ||120 ||TBD || |
|- | |- | ||
− | | 2|| | + | | 2||TBD||pBAD+RBS ||R/X;S/P ||R/X || ||K091001||lsrR ||R/X; S/P ||R/S ||954 ||TBD || |
|- | |- | ||
− | | 3|| | + | | 3||P0455 ||RBS+cI+TT ||R/X; S/P ||S/P ||896 ||TBD ||pBAD+RBS+lsrR ||R/X; S/P ||X/P || ||TBD || |
|- | |- | ||
− | | 4|| C0261||RBS+luxI ||R/X; S/P ||R/ | + | | 4|| K091106 ||pLsrA/cI ||R/X; S/P ||R/X || 141||C0261||RBS+luxI ||R/X; S/P ||R/S ||661 ||TBD|| |
+ | |S | ||
+ | | 5||B0015 ||TT ||R/X; S/P ||S/P ||129 ||I13025 ||RBS+Mnt ||R/X;S/P ||X/P ||331 ||TBD || | ||
|- | |- | ||
− | | | + | | 6||TBD ||RBS+Mnt+TT ||R/X; S/P ||S/P || ||TBD ||pLsrA/cI+RBS+luxI ||R/X; S/P ||X/P || || TBD|| |
|- | |- | ||
− | | | + | | 7|| TBD ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||S/P || ||TBD||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||X/P || ||TBD || |
− | |||
− | |||
|- | |- | ||
|} | |} | ||
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|- | |- | ||
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || | |[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || | ||
+ | |- | ||
+ | [http://partsregistry.org/Part:BBa_P0451 P0451]||RBS+cI+TT ||registry 07 ||Plt.1-21O ||pSB1AK3 || | ||
|- | |- | ||
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || | |[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || |
Revision as of 20:35, 2 July 2008
Goal:
pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT
I13453+B0034+K091001+P0455(B0034+C0051+B0015)+K091106+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015
Ligation Plan
Vector | Insert | Product | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Step | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Size | ||||||||||||||
1 | B0034 | RBS | R/X; S/P | S/P | 12 | I13453 | pBAD | R/X;S/P | X/P | 120 | TBD | |||||||||||||||
2 | TBD | pBAD+RBS | R/X;S/P | R/X | K091001 | lsrR | R/X; S/P | R/S | 954 | TBD | ||||||||||||||||
3 | P0455 | RBS+cI+TT | R/X; S/P | S/P | 896 | TBD | pBAD+RBS+lsrR | R/X; S/P | X/P | TBD | ||||||||||||||||
4 | K091106 | pLsrA/cI | R/X; S/P | R/X | 141 | C0261 | RBS+luxI | R/X; S/P | R/S | 661 | TBD | S | 5 | B0015 | TT | R/X; S/P | S/P | 129 | I13025 | RBS+Mnt | R/X;S/P | X/P | 331 | TBD | ||
6 | TBD | RBS+Mnt+TT | R/X; S/P | S/P | TBD | pLsrA/cI+RBS+luxI | R/X; S/P | X/P | TBD | |||||||||||||||||
7 | TBD | pLsrA/cI+RBS+luxI+RBS+Mnt+TT | R/X; S/P | S/P | TBD | pBAD+RBS+lsrR+RBS+cI+TT | R/X; S/P | X/P | TBD |
Parts
BBa_ | Description | Source | location('07) | Vector | Responsible |
---|---|---|---|---|---|
B0015 | double terminator | registry 07 | Plt.1-1I;Plt.3-3O | pSB1AK3 | |
B0034 | RBS | registry 07 | Plt.1-3O | pSB1A2 | |
I13453 | pBAD | registry 07 | Plt.2-13D; Plt.3-3I | pSB1A3 | |
C0261 | RBS+luxI | registry 07 | Plt.1-24A | pSB1A2 | |
P0455 | RBS+cI+TT | registry 07 | Plt.1-16M | pSB1AC3 | |
I13025 | RBS+Mnt | registry 07 | Plt.1-23J | pSB1A2 | |
K091001 | lsrR | MW | N/A | N/A | |
K091106 | plsrA/cI | MW | N/A | N/A |