Difference between revisions of "LsrA/cI"
From GcatWiki
| Line 1: | Line 1: | ||
'''Goal''': | '''Goal''': | ||
| − | '''pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT''' | + | '''pBAD+RBS+lsrK+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT''' |
| − | '''I13453'''+'''B0034'''+'''K091001'''+'''P0455'''(B0034+C0051+B0015)+'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015''' | + | '''I13453'''+'''B0034'''+'''K091002'''B0034'''+'''K091001'''+'''P0455'''(B0034+C0051+B0015)+'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015''' |
| Line 23: | Line 23: | ||
|- | |- | ||
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size''' | |'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size''' | ||
| + | |- | ||
| + | | 1|| B0034 ||RBS ||R/X; S/P||S/P || 12||K091001||lsrR ||R/X;S/P ||R/S ||120 ||TBD || | ||
|- | |- | ||
| 1|| B0034 ||RBS ||R/X; S/P||R/X || 12||I13453||pBAD ||R/X;S/P ||R/S ||120 ||TBD || | | 1|| B0034 ||RBS ||R/X; S/P||R/X || 12||I13453||pBAD ||R/X;S/P ||R/S ||120 ||TBD || | ||
|- | |- | ||
| − | | 2||TBD||pBAD+RBS ||R/X;S/P ||S/P || || | + | | 2||TBD||pBAD+RBS ||R/X;S/P ||S/P || ||K091002||lsrK ||R/X; S/P ||X/P ||954 ||TBD || |
|- | |- | ||
| 3||P0455 ||RBS+cI+TT ||R/X; S/P ||R/X ||896 ||TBD ||pBAD+RBS+lsrR ||R/X; S/P ||R/S || ||TBD || | | 3||P0455 ||RBS+cI+TT ||R/X; S/P ||R/X ||896 ||TBD ||pBAD+RBS+lsrR ||R/X; S/P ||R/S || ||TBD || | ||
Revision as of 18:53, 8 July 2008
Goal:
pBAD+RBS+lsrK+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT
I13453+B0034+K091002B0034+K091001+P0455(B0034+C0051+B0015)+K091106+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015
Ligation Plan
| Vector | Insert | Product | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Step | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Size |
| 1 | B0034 | RBS | R/X; S/P | S/P | 12 | K091001 | lsrR | R/X;S/P | R/S | 120 | TBD | |
| 1 | B0034 | RBS | R/X; S/P | R/X | 12 | I13453 | pBAD | R/X;S/P | R/S | 120 | TBD | |
| 2 | TBD | pBAD+RBS | R/X;S/P | S/P | K091002 | lsrK | R/X; S/P | X/P | 954 | TBD | ||
| 3 | P0455 | RBS+cI+TT | R/X; S/P | R/X | 896 | TBD | pBAD+RBS+lsrR | R/X; S/P | R/S | TBD | ||
| 4 | K091106 | pLsrA/cI | R/X; S/P | S/P | 141 | C0261 | RBS+luxI | R/X; S/P | X/P | 661 | TBD | |
| 5 | B0015 | TT | R/X; S/P | R/X | 129 | I13025 | RBS+Mnt | R/X;S/P | R/S | 331 | TBD | |
| 6 | TBD | RBS+Mnt+TT | R/X; S/P | R/X | TBD | pLsrA/cI+RBS+luxI | R/X; S/P | R/S | TBD | |||
| 7 | TBD | pLsrA/cI+RBS+luxI+RBS+Mnt+TT | R/X; S/P | R/X | TBD | pBAD+RBS+lsrR+RBS+cI+TT | R/X; S/P | R/S | TBD |
Parts
| BBa_ | Description | Source | location('07) | Vector | Responsible |
|---|---|---|---|---|---|
| B0015 | double terminator | registry 07 | Plt.1-1I;Plt.3-3O | pSB1AK3 | |
| B0034 | RBS | registry 07 | Plt.1-3O | pSB1A2 | |
| I13453 | pBAD | registry 07 | Plt.2-13D; Plt.3-3I | pSB1A3 | |
| C0261 | RBS+luxI | registry 07 | Plt.1-24A | pSB1A2 | |
| P0455 | RBS+cI+TT | registry 07 | Plt.1-16M | pSB1AC3 | |
| I13025 | RBS+Mnt | registry 07 | Plt.1-23J | pSB1A2 | |
| K091001 | lsrR | MW | N/A | N/A | |
| K091106 | plsrA/cI | MW | N/A | N/A |
