Difference between revisions of "LsrA/cI"
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| 5||TBD||RBS+lsrR+TT||R/X; S/P ||R/X || ||TBD ||pBAD+RBA+lsrK ||R/X; S/P ||R/S || ||TBD || | | 5||TBD||RBS+lsrR+TT||R/X; S/P ||R/X || ||TBD ||pBAD+RBA+lsrK ||R/X; S/P ||R/S || ||TBD || | ||
|- | |- | ||
| − | | 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141+2079||C0261||RBS+luxI ||R/X; S/P ||X/P ||661 || | + | | 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141+2079||C0261||RBS+luxI ||R/X; S/P ||X/P ||661 ||K091140||810 |
|- | |- | ||
| 7||B0015 ||TT ||R/X; S/P ||R/X ||129+2079 ||I13025 ||RBS+Mnt ||R/X;S/P ||R/S ||331 ||TBD || | | 7||B0015 ||TT ||R/X; S/P ||R/X ||129+2079 ||I13025 ||RBS+Mnt ||R/X;S/P ||R/S ||331 ||TBD || | ||
Revision as of 18:05, 15 July 2008
Goal:
pBAD+RBS+lsrK+RBS+lsrR+TT
pLsrA/cI+RBS+LuxI+RBS+Mnt+TT
I13453+B0034+K091002+B0034+K091001+B0015
K091106+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015
Ligation Plan
| Vector | Insert | Product | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Step | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Size |
| 1 | B0034 | RBS | R/X; S/P | S/P | 12+2079 | K091001 | lsrR | R/X;S/P | X/P | 954 | K091138 | 972 |
| 2 | B0034 | RBS | R/X; S/P | S/P | 12+2079 | K091002 | lsrK | R/X;S/P | X/P | 1593 | TBD | |
| 3 | I13453 | pBAD | R/X;S/P | S/P | 120+2079 | TBD | RBS+lsrK | R/X; S/P | X/P | TBD | ||
| 4 | B0015 | TT | R/X; S/P | R/X | 129+2079 | TBD | RBS+lsrR | R/X; S/P | R/S | K091139 | 1109 | |
| 5 | TBD | RBS+lsrR+TT | R/X; S/P | R/X | TBD | pBAD+RBA+lsrK | R/X; S/P | R/S | TBD | |||
| 6 | K091106 | pLsrA/cI | R/X; S/P | S/P | 141+2079 | C0261 | RBS+luxI | R/X; S/P | X/P | 661 | K091140 | 810 |
| 7 | B0015 | TT | R/X; S/P | R/X | 129+2079 | I13025 | RBS+Mnt | R/X;S/P | R/S | 331 | TBD | |
| 8 | TBD | RBS+Mnt+TT | R/X; S/P | R/X | TBD | pLsrA/cI+RBS+luxI | R/X; S/P | R/S | TBD |
Parts
| BBa_ | Description | Source | location('07) | Vector | Responsible |
|---|---|---|---|---|---|
| B0015 | double terminator | registry 07 | Plt.1-1I;Plt.3-3O | pSB1AK3 | |
| B0034 | RBS | registry 07 | Plt.1-3O | pSB1A2 | |
| I13453 | pBAD | registry 07 | Plt.2-13D; Plt.3-3I | pSB1A3 | |
| C0261 | RBS+luxI | registry 07 | Plt.1-24A | pSB1A2 | |
| I13025 | RBS+Mnt | registry 07 | Plt.1-23J | pSB1A2 | |
| K091001 | lsrR | MW | N/A | N/A | |
| K091002 | lsrK | MW | N/A | N/A | |
| K091106 | plsrA/cI | MW | N/A | N/A |
