Difference between revisions of "LsrA/cI"

From GcatWiki
Jump to: navigation, search
Line 36: Line 36:
 
| 4||B0015||TT||R/X; S/P ||R/X ||129+2079 ||TBD ||RBS+lsrR  ||R/X; S/P  ||R/S  || ||K091139 ||1109
 
| 4||B0015||TT||R/X; S/P ||R/X ||129+2079 ||TBD ||RBS+lsrR  ||R/X; S/P  ||R/S  || ||K091139 ||1109
 
|-
 
|-
| 5||TBD||RBS+lsrR+TT||R/X; S/P ||R/X || ||TBD ||pBAD+RBA+lsrK  ||R/X; S/P  ||R/S  || ||TBD ||
+
| 5||K091139||RBS+lsrR+TT||R/X; S/P ||R/X ||1109+2079 ||TBD ||pBAD+RBA+lsrK  ||R/X; S/P  ||R/S  || ||TBD ||
 
|-
 
|-
 
| 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141+2079||C0261||RBS+luxI  ||R/X; S/P  ||X/P  ||661 ||K091140||810
 
| 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141+2079||C0261||RBS+luxI  ||R/X; S/P  ||X/P  ||661 ||K091140||810
 
|-
 
|-
| 7||B0015 ||TT ||R/X; S/P ||R/X ||129+2079 ||I13025 ||RBS+Mnt  ||R/X;S/P  ||R/S  ||331 ||TBD ||
+
| 7||B0015 ||TT ||R/X; S/P ||R/X ||129+2079 ||I13025 ||RBS+Mnt  ||R/X;S/P  ||R/S  ||331 ||K091141 ||468
 
|-
 
|-
| 8||TBD ||RBS+Mnt+TT ||R/X; S/P ||R/X || ||TBD ||pLsrA/cI+RBS+luxI  ||R/X; S/P  ||R/S  || || TBD||
+
| 8||K091141||RBS+Mnt+TT ||R/X; S/P ||R/X || 468||K091140 ||pLsrA/cI+RBS+luxI  ||R/X; S/P  ||R/S  ||810 || TBD||
 
|-
 
|-
 
|}
 
|}

Revision as of 18:09, 15 July 2008

Goal:

pBAD+RBS+lsrK+RBS+lsrR+TT

pLsrA/cI+RBS+LuxI+RBS+Mnt+TT

I13453+B0034+K091002+B0034+K091001+B0015

K091106+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015


Ligation Plan

Vector Insert Product
Step BBa_ Description Pre-/suffix Enzymes Size BBa_ Description Pre-/suffix Enzymes Size BBa_ Size
1 B0034 RBS R/X; S/P S/P 12+2079 K091001 lsrR R/X;S/P X/P 954 K091138 972
2 B0034 RBS R/X; S/P S/P 12+2079 K091002 lsrK R/X;S/P X/P 1593 TBD
3 I13453 pBAD R/X;S/P S/P 120+2079 TBD RBS+lsrK R/X; S/P X/P TBD
4 B0015 TT R/X; S/P R/X 129+2079 TBD RBS+lsrR R/X; S/P R/S K091139 1109
5 K091139 RBS+lsrR+TT R/X; S/P R/X 1109+2079 TBD pBAD+RBA+lsrK R/X; S/P R/S TBD
6 K091106 pLsrA/cI R/X; S/P S/P 141+2079 C0261 RBS+luxI R/X; S/P X/P 661 K091140 810
7 B0015 TT R/X; S/P R/X 129+2079 I13025 RBS+Mnt R/X;S/P R/S 331 K091141 468
8 K091141 RBS+Mnt+TT R/X; S/P R/X 468 K091140 pLsrA/cI+RBS+luxI R/X; S/P R/S 810 TBD

Parts

BBa_ Description Source location('07) Vector Responsible
B0015 double terminator registry 07 Plt.1-1I;Plt.3-3O pSB1AK3
B0034 RBS registry 07 Plt.1-3O pSB1A2
I13453 pBAD registry 07 Plt.2-13D; Plt.3-3I pSB1A3
C0261 RBS+luxI registry 07 Plt.1-24A pSB1A2
I13025 RBS+Mnt registry 07 Plt.1-23J pSB1A2
K091001 lsrR MW N/A N/A
K091002 lsrK MW N/A N/A
K091106 plsrA/cI MW N/A N/A