LsrA/cI

Goal: pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT

I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015

Ligation Plan

	Vector					Insert					Product
Step	BBa_	Description	Pre-/suffix	Enzymes	Size	BBa_	Description	Pre-/suffix	Enzymes	Size	BBa_	Size
1	I13453	pBAD	R/X;S/P	S/P	120	B0034	RBS	R/X; S/P	X/P	12
2	K091001	lsrR	R/X; S/P	R/X	954		pBAD+RBS	R/X;S/P	R/S
3		pBAD+RBS+lsrR	R/X; S/P	S/P		P0455	RBS+cI+TT	R/X; S/P	X/P	896
4	C0261	RBS+luxI	R/X; S/P	R/X	661	K091106	pLsrA/cI	R/X; S/P	R/S	141
5	I13025	RBS+Mnt	R/X;S/P	S/P	331	B0015	TT	R/X; S/P	X/P	129
6		pLsrA/cI+RBS+luxI	R/X; S/P	S/P			RBS+Mnt+TT	R/X; S/P	X/P
7		pBAD+RBS+lsrR+RBS+cI+TT	R/X; S/P	S/P			pLsrA/cI+RBS+luxI+RBS+Mnt+TT	R/X; S/P	X/P

Parts

BBa_	Description	Source	Responsible	location('07)	Vector
B0015	double terminator	registry 07	Plt.1-1I;Plt.3-3O	pSB1AK3
B0034	RBS	registry 07	Plt.1-3O	pSB1A2
I13453	pBAD	registry 07	Plt.2-13D; Plt.3-3I	pSB1A3
C0261	RBS+luxI	registry 07	Plt.1-24A	pSB1A2
P0455	RBS+cI+TT	registry 07	Plt.1-16M	pSB1AC3