LsrA/cI

Goal:

pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT

I13453+B0034+K091001+P0455(B0034+C0051+B0015)+K091106+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015

Ligation Plan

	Vector					Insert					Product
Step	BBa_	Description	Pre-/suffix	Enzymes	Size	BBa_	Description	Pre-/suffix	Enzymes	Size	BBa_	Size
1	B0034	RBS	R/X; S/P	S/P	12	I13453	pBAD	R/X;S/P	X/P	120	TBD
2	TBD	pBAD+RBS	R/X;S/P	R/X		K091001	lsrR	R/X; S/P	R/S	954	TBD
3	P0455	RBS+cI+TT	R/X; S/P	S/P	896	TBD	pBAD+RBS+lsrR	R/X; S/P	X/P		TBD
4	K091106	pLsrA/cI	R/X; S/P	R/X	141	C0261	RBS+luxI	R/X; S/P	R/S	661	TBD		S	5	B0015	TT	R/X; S/P	S/P	129	I13025	RBS+Mnt	R/X;S/P	X/P	331	TBD
6	TBD	RBS+Mnt+TT	R/X; S/P	S/P		TBD	pLsrA/cI+RBS+luxI	R/X; S/P	X/P		TBD
7	TBD	pLsrA/cI+RBS+luxI+RBS+Mnt+TT	R/X; S/P	S/P		TBD	pBAD+RBS+lsrR+RBS+cI+TT	R/X; S/P	X/P		TBD

Parts

P0451||RBS+cI+TT ||registry 07 ||Plt.1-21O ||pSB1AK3 ||

BBa_	Description	Source	location('07)	Vector	Responsible
B0015	double terminator	registry 07	Plt.1-1I;Plt.3-3O	pSB1AK3
B0034	RBS	registry 07	Plt.1-3O	pSB1A2
I13453	pBAD	registry 07	Plt.2-13D; Plt.3-3I	pSB1A3
C0261	RBS+luxI	registry 07	Plt.1-24A	pSB1A2
P0455	RBS+cI+TT	registry 07	Plt.1-16M	pSB1AC3
I13025	RBS+Mnt	registry 07	Plt.1-23J	pSB1A2
K091001	lsrR	MW	N/A	N/A
K091106	plsrA/cI	MW	N/A	N/A