LsrA/cI

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Goal:

pBAD+RBS+lsrK+RBS+lsrR+TT

pLsrA/cI+RBS+LuxI+RBS+Mnt+TT

I13453+B0034+K091002+B0034+K091001+B0015

K091106+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015


Ligation Plan

Vector Insert Product
Step BBa_ Description Pre-/suffix Enzymes Size BBa_ Description Pre-/suffix Enzymes Size BBa_ Size
1 B0034 RBS R/X; S/P S/P 12+2079 K091001 lsrR R/X;S/P X/P 954 TBD
2 B0034 RBS R/X; S/P S/P 12+2079 K091002 lsrK R/X;S/P X/P 1593 TBD
3 I13453 pBAD R/X;S/P S/P 120+2079 TBD RBS+lsrK R/X; S/P X/P TBD
4 B0015 TT R/X; S/P R/X 129+2079 TBD RBS+lsrR R/X; S/P R/S TBD
5 TBD RBS+lsrR+TT R/X; S/P R/X TBD pBAD+RBA+lsrK R/X; S/P R/S TBD
6 K091106 pLsrA/cI R/X; S/P S/P 141+2079 C0261 RBS+luxI R/X; S/P X/P 661 TBD
7 B0015 TT R/X; S/P R/X 129+2079 I13025 RBS+Mnt R/X;S/P R/S 331 TBD
8 TBD RBS+Mnt+TT R/X; S/P R/X TBD pLsrA/cI+RBS+luxI R/X; S/P R/S TBD

Parts

P0451||RBS+cI+TT ||registry 07 ||Plt.1-21O ||pSB1AK3 ||
BBa_ Description Source location('07) Vector Responsible
B0015 double terminator registry 07 Plt.1-1I;Plt.3-3O pSB1AK3
B0034 RBS registry 07 Plt.1-3O pSB1A2
I13453 pBAD registry 07 Plt.2-13D; Plt.3-3I pSB1A3
C0261 RBS+luxI registry 07 Plt.1-24A pSB1A2
P0455 RBS+cI+TT registry 07 Plt.1-16M pSB1AC3
I13025 RBS+Mnt registry 07 Plt.1-23J pSB1A2
K091001 lsrR MW N/A N/A
K091002 lsrK MW N/A N/A
K091106 plsrA/cI MW N/A N/A