MWSU Research

May 21, 2014

The article by Seffernick et al. shows how researchers inserted melamine deaminase, triA gene, into E. coli. TriA was cloned into the EcoRI and HindIII sites of pUC18, and the resulting plasmid pJS3 was transformed into E. coli. When melamine is present, the gene will result in the many products including ammeline and ammelide; however, the production of ammelide and other products is insignificant.

This figure shows the decreasing concentration (mM) of melamine as ammeline and ammelide are produced over the course of 15 minutes.

References

Seffernick JL, de Souza ML, Sadowsky MJ, Wackett LP. 2001. Melamine deaminase and atrazine chlorohydrolase: 98 percent identical but functionally different. J. Bacteriol. 183:2405–2410