Difference between revisions of "MWSU protocols"

@@ Line 1: / Line 1: @@
-[[What to do with a new clone]]
+'''Purification of DNA'''
+[[Isolation of Genomic DNA from Bacteria]]
-[[Diagnostic RP Digestion for Checking Insert Size]]
+'''PCR'''
-[[DNA Sequencing at Iowa State University]]
+[[Standard PCR]]
-[[BioBrick Digestions for Fragment and Vector Preparation]]
+[[Resuspending Oligos]]
-[[Reducing Background from Double Digested Vector]]
+'''Recombinant DNA Production'''
 [[Pouring an Agarose Gel]]
+[[BioBrick Digestions for Fragment and Vector Preparation]]
 [[Fragment Purification]]
+[[Direct Synthesis with Overlapping Oligos]]
 [[Ethanol Precipitation of Vector DNA]]
+[[Reducing Background from Double Digested Vector]]
+'''Ligation and Transformation'''
 [[BioBrick Ligations]]
@@ Line 19: / Line 29: @@
 [[Ligation and Transformation]]
-[[Davidson_Missouri_W/colony_PCR | Colony PCR]]
+[[Bacterial Media]]
+'''Screening Clones'''
-[[Standard PCR]]
+[[Diagnostic RP Digestion for Checking Insert Size]]
-[[Isolation of Genomic DNA from Bacteria]]
+[[Davidson_Missouri_W/colony_PCR | Colony PCR]]
-[[Resuspending Oligos]]
+[[DNA Sequencing at Iowa State University]]
-[[Direct Synthesis with Overlapping Oligos]]
+[[What to do with a new clone]]
-[[Bacterial Media]]
+'''Measuring Phenotypes'''
 [[Measuring Fluorescence in Bacteria]]

Revision as of 13:29, 30 June 2011