Difference between revisions of "MWSU protocols"

Revision as of 21:23, 6 March 2013

Purification of DNA Isolation of Genomic DNA from Bacteria

PCR

Standard PCR

Resuspending Oligos

Colony PCR

Recombinant DNA Production

Golden Gate Assembly Protocol for BsmB1

Pouring an Agarose Gel

BioBrick Digestions for Fragment and Vector Preparation

Fragment Purification

Gibson Assembly

Direct Synthesis with Overlapping Oligos

Annealing Oligos for Cloning

Ethanol Precipitation of Vector DNA

Reducing Background from Double Digested Vector

Ligation and Transformation

BioBrick Ligations

Ligation and Transformation

Bacterial Media

Screening Clones

Diagnostic RP Digestion for Checking Insert Size

DNA Sequencing at Iowa State University

What to do with a new clone

Measuring Phenotypes

Measuring Fluorescence in Bacteria