March 22, 2016
sample dendrograms
- We want correct clusters through dendrogram.
normalizations
- DEseq doesn't care how long the gene is. The only normalization we are doing is how similar the genes are. Normalization through Deseq is only taking into account "how many genes per sample" DEseq only compares same gene against itself.
- normalized accounts asks software how many reads.
one single sample can throw off the dendrogram
Do you think the signal for liver will be different for liver?
-Not necessarily, so crosscheck your list with some groups from other organs!
83 genes on list of clustering done correctly from 3/22
SGTA- co-chaperone that specializes in hormone receptors- helps fold transcription factors that help receptors
Look at the story that you are telling! Modifying proteins like receptors, signaling pathways are starting.
Today we continued looking for GO terms and KEGG pathway identifiers for our 31 genes unique to our list. We also clustered correctly and got 83 genes. Then we researched the function of each gene and checked to see if the genes were up or down regulated. Down regulated genes are highlighted in blue.
GENES DR. CAMPBELL GOT EXCITED ABOUT: LGR5, ZFP161
