Difference between revisions of "Mnt/Lac"

Latest revision as of 21:20, 2 July 2008

Parts Relevant to XOR Gate:

part no.	description	source	responsible
K091104	pMnt/Lac	MW '08	AA
B0034	RBS	registry	"
P0455	RBS+cI+TT	registry	"
K091109	LuxS +AI-2	MW '08	"
C0012	LacI (+LVA)	DC '08	"
B0015	TT	registry	"
I13453	pBAD	registry	"

Goal:

pBAD+RBS+LacI+TT+pMnt/Lac+RBS+LuxS+RBS+cI+TT

I13453 + B0034+ C0012 + B0015 + K091104 + B0034 + K091109 + P0455 (B0034+C0051+B0015)

Ligation Plan

	Vector					Insert					Product
Step	BBa_	Description	Pre-/suffix	Enzymes	Size bp	BBa_	Description	Pre-/suffix	Enzymes	Size bp	BBa_	Size
1	B0034	RBS	R/X; S/P	S/P	12	I13453	pBAD	R/X;S/P	X/P	120	TBD
2	TBD	pBAD+RBS	R/X;S/P	R/X		C0012	LacI	R/X; S/P	R/S	1128	TBD
3	TBD	pBAD+RBS+LacI	R/X; S/P	S/P	1128	B0015	TT	R/X; S/ P	X/P	129	TBD
4	TBD	pBAD+RBS+LacI+TT	R/X; S/P	R/X	TBD	K091104	pMnt/Lac	R/X; S/P	R/S	87	TBD
5	TBD	pBAD+RBS+LacI+TT+pMnt/Lac	R/X; S/P	S/P	TBD	B0034	RBS	R/X;S/P	R/S	12	TBD
6	TBD	pBAD+RBS+LacI+TT+pMnt/Lac+RBS	R/X; S/P	S/P	TBD	K091109	LuxS	R/X; S/P	X/P	516	TBD
7	TBD	pBAD+RBS+LacI+TT+pMnt/Lac+RBS+LuxS	R/X; S/P	S/P	TBD	P0455	RBS+cI+TT	R/X; S/P	X/P	896	TBD

@@ Line 29: / Line 29: @@
 '''I13453 + B0034+ C0012 + B0015 + K091104 + B0034 + K091109 + P0455''' (B0034+C0051+B0015)
+'''Ligation Plan'''
+{| border="1" cellpadding="2"
+!width="20"|
+!width="30"|Vector
+!width="110"|
+!width="30"|
+!width="30"|
+!width="30"|
+!width="30"|Insert
+!width="110"|
+!width="30"|
+!width="30"|
+!width="30"|
+!width="30"|Product
+!width="30"|
+|-
+|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size bp'''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size bp'''||'''BBa_'''||'''Size'''
+|-
+| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12||I13453||pBAD  ||R/X;S/P  ||X/P  ||120 ||TBD ||
+|-
+| 2||TBD||pBAD+RBS ||R/X;S/P ||R/X || ||C0012||LacI ||R/X; S/P  ||R/S  ||1128 ||TBD ||
+|-
+| 3||TBD||pBAD+RBS+LacI ||R/X; S/P ||S/P ||1128 ||B0015 ||TT  ||R/X; S/ P  ||X/P  ||129 ||TBD ||
+|-
+| 4|| TBD ||pBAD+RBS+LacI+TT ||R/X; S/P ||R/X || TBD||K091104||pMnt/Lac  ||R/X; S/P  ||R/S  ||87 ||TBD||
+|-
+| 5|| TBD||pBAD+RBS+LacI+TT+pMnt/Lac ||R/X; S/P ||S/P ||TBD ||B0034 ||RBS  ||R/X;S/P  ||R/S  || 12 ||TBD ||
+|-
+| 6||TBD ||pBAD+RBS+LacI+TT+pMnt/Lac+RBS ||R/X; S/P ||S/P ||TBD || K091109 ||LuxS  ||R/X; S/P  ||X/P  ||516 || TBD||
+|-
+| 7|| TBD ||pBAD+RBS+LacI+TT+pMnt/Lac+RBS+LuxS ||R/X; S/P ||S/P ||TBD ||P0455||RBS+cI+TT  ||R/X; S/P  ||X/P  ||896 ||TBD ||
+|-
+|}