Difference between revisions of "Test XOR Hybrid Promoters"

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[[Test pLsrR/cI]]
 
[[Test pLsrR/cI]]
  
pLsrR/cI is constitutively on and is repressed by LsrR. The addition of AI-2, which becomes phosphorylated by LsrK, represses LsrR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off.
+
Test pLasR/cI
  
Some promoter→LsrK+LsrR+t+t+pLsrR/cI→GFP  does not glow
+
pLasR/cI is constitutively off and is repressed by cI. The addition of p-AI-1, activates LasR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off.
 
 
 
 
add AI-2             
 
                                               
 
Some promoter→LsrK+LsrR+t+t+pLsrR/cI→GFP glows
 
 
 
 
 
add AI-2
 
                                                             
 
Some promoter→LsrK+LsrR+cI+t+t+pLsrR/cI→GFP does not glows
 
  
 
{| border="1" cellpadding="2"
 
{| border="1" cellpadding="2"
!width="30"|LsrR
+
!width="30"|LasR
 
!width="30"|cI
 
!width="30"|cI
!width="30"|AI-2 + GFP
+
!width="30"|p-AI-1 + GFP
!width="30"|AI-2 - GFP
+
!width="30"|p-AI-1 - GFP
 
|-
 
|-
| 0 || 0 || 1 || 1
+
| 0 || 0 || 0 || 0
 
|-
 
|-
 
| 1 || 0 || 1 || 0  
 
| 1 || 0 || 1 || 0  

Revision as of 20:03, 30 June 2008

This is a rough idea of how to test the four hybrid promoters for the XOR gate.


Test pLuxR/cI


Test pMnt/Lac

pMnt/Lac

pMnt/Lac is constitutively on but is repressed by the LacI protein. The promoter is also repressed by the Mnt protein. The addition of IPTG should repress the LacI repressor and glow in the absence of the Mnt protein.

Some promoter→LacI+t+t+pLac/Mnt→GFP does not glow

Some promoter→Mnt+t+t+pLac/Mnt→GFP does not glow


add IPTG

Some promoter→LacI+t+t+pLac/Mnt→GFP glows


LacI Mnt IPTG + GFP IPTG - GFP
0 0 1 1
0 1 0 0
1 0 1 0
1 1 0 0



Test pLsrR/cI

Test pLasR/cI

pLasR/cI is constitutively off and is repressed by cI. The addition of p-AI-1, activates LasR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off.

LasR cI p-AI-1 + GFP p-AI-1 - GFP
0 0 0 0
1 0 1 0
0 1 0 0
1 1 0 0



Test Mnt/Tet promoter

The pMnt/TetR promoter is constitutively on and is repressed by the TetR protein. The addition of aTc should repress the TetR causing it to glow. The addition of the Mnt protein should repress the promoter causing it not glow.


TetR Mnt aTc + GFP aTc - GFP
0 0 1 1
0 1 0 0
1 0 1 0
1 1 0 0


Test tet repression


Some promoter (pbad)→RBS+TetR+t+t+pMnt/Tet→RBS+GFP does not glows

I13453+RBS+C0040+TT+K091105+E0240


add aTc

Some promoter→TetR+t+t+pMnt/Tet→GFP glows

I13453+RBS+C0040+TT+K091105+E0240


Test Mnt repression:

Some promoter→Mnt+t+t+pMnt/Tet→GFP glows

I13453+RBS+C0072+TT+K091105+E0240


Test Tet and Mnt repression:

Some promoter→TetR+Mnt+t+t+pMnt/Tet→GFP does not glows

I13453+RBS+C0040+C0072+TT+K091105+E0240

add aTc does not glow