Server at Davidson College



The Oligator
A program designed to choose oligos for DNA sequences of most lengths
If the Oligator does not produce a satisfactory set of Oligos, or if you want to specify particular oligos, consider using the Do-It-Yourself Oligator.
*Note: This site is optimized for Safari and Firefox. Chrome and IE are NOT supported.
*Note: U's will automatically be converted to T's
Enter DNA sequence between 15 and 20,000 base pairs (5' to 3')


Oligo Lengths: 
Overlap Lengths:     
Note: Minimum Length is 15.
     
Add Golden Gate Assembly Sticky Ends  Add BioBrick Prefix and Suffix 
 
Top left strand:  5' 3'  
Bottom right strand:  3' 5'  top strand   
Golden Gate Enzyme:  bottom strand  
 
  top strand  
  bottom strand
  Check for BioBrick Restriction Sites 
  Remove BioBrick Restriction Sites 
  ORF begins at base
     
*Note: This site is optimized for Safari and Firefox. Chrome and IE are NOT supported.