Server at Davidson College



The Oligator
A program designed to choose oligos for DNA sequences of most lengths

Enter DNA sequence between 22 and 20,000 base pairs (5' to 3')


     Oligo Lengths:   to  Enter a tag for sequence (optional):   
Overlap Lengths:   to     
     
Add Golden Gate Assembly Sticky Ends  Add BioBrick Prefix and Suffix 
 
Top left strand:  5' 3'  
Bottom right strand:  3' 5'  top strand   
Golden Gate Enzyme:  bottom strand  
 
  top strand  
  bottom strand
  Check for BioBrick Restriction Sites 
  Remove BioBrick Restriction Sites 
  ORF begins at base