Difference between revisions of "Davidson Missouri W/MWSU protocols"
From GcatWiki
(4 intermediate revisions by the same user not shown) | |||
Line 2: | Line 2: | ||
[[Diagnostic RP Digestion for Checking Insert Size]] | [[Diagnostic RP Digestion for Checking Insert Size]] | ||
+ | |||
+ | [[DNA Sequencing at Iowa State University]] | ||
[[BioBrick Digestions for Fragment and Vector Preparation]] | [[BioBrick Digestions for Fragment and Vector Preparation]] | ||
+ | |||
+ | [[Reducing Background from Double Digested Vector]] | ||
+ | |||
+ | [[Pouring an Agarose Gel]] | ||
[[Fragment Purification]] | [[Fragment Purification]] | ||
+ | |||
+ | [[Ethanol Precipitation of Vector DNA]] | ||
+ | |||
+ | [[BioBrick Ligations]] | ||
[[Ligation and Transformation]] | [[Ligation and Transformation]] | ||
Line 12: | Line 22: | ||
[[Standard PCR]] | [[Standard PCR]] | ||
+ | |||
+ | [[Isolation of Genomic DNA from Bacteria]] | ||
[[Resuspending Oligos]] | [[Resuspending Oligos]] |
Latest revision as of 14:38, 8 September 2010
Diagnostic RP Digestion for Checking Insert Size
DNA Sequencing at Iowa State University
BioBrick Digestions for Fragment and Vector Preparation
Reducing Background from Double Digested Vector
Ethanol Precipitation of Vector DNA
Isolation of Genomic DNA from Bacteria