DNA Sequencing at Iowa State University

Prepare minipreps of DNA to be sequenced – concentration should be at least 50 ng/ul
Put 10 ul of each DNA template in a 1.5 ml tube
Label the DNA template tubes with a name not more than 8 characters
Seal tubes with parafilm and place in 50 ml tube
Wrap in bubblewrap
Go to http://www.dna.iastate.edu/users/index.html
Login = eckdahl, Password = toad
Input number of reactions, choose Plasmid Template Type, hit Continue
Input names of template exactly as written on tubes
Input concentration in ug/ul (eg. 50 ng/ul = .050 ug/ul)
Input approximate insert size and approximate vector size
Input G00100 for Forward primer or G00101 for Reverse primer
Input “Eckdahl primer box” under Comments
Press Continue
Address Error Message if you can
Press Submit
Print page with Samples Submitted and Tracking Number
Place page and 50 tube(s) with DNA templates in FedEx box for shipping to

  DNA Sequencing Facility, Iowa State University, 1184 Molecular Biology Bldg, Ames, IA, 50011-3260

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