Difference between revisions of "Biology"
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| + | [[Davidson Protocols]] <br> | ||
| + | [[MWSU_protocols]] <br> | ||
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| + | [[J-GGA Scaffold Design]] | ||
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| + | [[J-GGA Scaffold 2.0 Design]] | ||
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| + | [[Origin of Replication]] | ||
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| + | [[File:Gelpouring.jpg|200px|thumb|right|Look, Mom, no EtBr!]] | ||
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| + | [//gcat.davidson.edu/SynBio13/primer GGA Primer Designer] | ||
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'''Topics to Investigate''' | '''Topics to Investigate''' | ||
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| + | '''Family of Xanthine compounds''' | ||
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| + | [[File:Caffeine_demethylase_word_doc.docx]] | ||
<u>High Priority = more people</u> | <u>High Priority = more people</u> | ||
| − | * eCDM8 working to make theophylline with biosensor and fitness modules (Tet | + | * '''Phoebe and Claire:''' eCDM8 working to make theophylline with biosensor and fitness modules (Tet<sup>R</sup>) to report out. <br> |
Which plasmid carries which device?<br> | Which plasmid carries which device?<br> | ||
| − | * Determine the junction sequences and associated primers<br> | + | Biosensor<br> |
| + | Fitness (tetA)<br> | ||
| + | feeds into stress module<br> | ||
| + | * '''Sachii and Jess:''' Determine the junction sequences and associated primers<br> | ||
(use existing rules)<br> | (use existing rules)<br> | ||
build scaffold for insert segments<br> | build scaffold for insert segments<br> | ||
| Line 11: | Line 33: | ||
include drug resistance gene<br> | include drug resistance gene<br> | ||
test JGG design<br> | test JGG design<br> | ||
| − | * test fitness module with adhE | + | * '''Spencer and Sara:''' test fitness module with adhE |
compare with tetA<br> | compare with tetA<br> | ||
fine tune tetA resistance<br> | fine tune tetA resistance<br> | ||
combine with tetA<br> | combine with tetA<br> | ||
Does theophylline diffuse across the membrane and thus the system fails?<br> | Does theophylline diffuse across the membrane and thus the system fails?<br> | ||
| − | * Copy number of plasmids <br> | + | * '''Brandon and Erich:''' Copy number of plasmids <br> |
Can we alter Ori to produce a range of plasmid densities in ''E. coli''? <br> | Can we alter Ori to produce a range of plasmid densities in ''E. coli''? <br> | ||
Latest revision as of 16:58, 30 July 2013
Davidson Protocols
MWSU_protocols
Topics to Investigate
Family of Xanthine compounds
File:Caffeine demethylase word doc.docx
High Priority = more people
- Phoebe and Claire: eCDM8 working to make theophylline with biosensor and fitness modules (TetR) to report out.
Which plasmid carries which device?
Biosensor
Fitness (tetA)
feeds into stress module
- Sachii and Jess: Determine the junction sequences and associated primers
(use existing rules)
build scaffold for insert segments
include orI (collaborate with copy number research)
include drug resistance gene
test JGG design
- Spencer and Sara: test fitness module with adhE
compare with tetA
fine tune tetA resistance
combine with tetA
Does theophylline diffuse across the membrane and thus the system fails?
- Brandon and Erich: Copy number of plasmids
Can we alter Ori to produce a range of plasmid densities in E. coli?
Second Order Priority = one person
- produce different CDM alleles
- swap out different components (promoters, RBS, alleles)
- test out programmed evolution
- Stress module needs more work (see eCDM8 outputs)
