Biology

Davidson Protocols
MWSU_protocols

J-GGA Scaffold Design

J-GGA Scaffold 2.0 Design

Origin of Replication

Look, Mom, no EtBr!

GGA Primer Designer

Topics to Investigate

Family of Xanthine compounds

File:Caffeine demethylase word doc.docx

High Priority = more people

• Phoebe and Claire: eCDM8 working to make theophylline with biosensor and fitness modules (Tet^R) to report out.
  

Which plasmid carries which device?
Biosensor
Fitness (tetA)
feeds into stress module

• Sachii and Jess: Determine the junction sequences and associated primers
  

(use existing rules)
build scaffold for insert segments
include orI (collaborate with copy number research)
include drug resistance gene
test JGG design

• Spencer and Sara: test fitness module with adhE

compare with tetA
fine tune tetA resistance
combine with tetA
Does theophylline diffuse across the membrane and thus the system fails?

• Brandon and Erich: Copy number of plasmids
  

Can we alter Ori to produce a range of plasmid densities in E. coli?

Second Order Priority = one person

• produce different CDM alleles
• swap out different components (promoters, RBS, alleles)
• test out programmed evolution
• Stress module needs more work (see eCDM8 outputs)