Difference between revisions of "DNA Sequencing at Iowa State University"
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| − | + | * Prepare minipreps of DNA to be sequenced – concentration should be at least 50 ng/ul | |
| − | + | * Put 10 ul of each DNA template in a 1.5 ml tube | |
| − | + | * Label the DNA template tubes with a name not more than 8 characters | |
| − | + | * Seal tubes with parafilm and place in 50 ml tube | |
| − | + | * Wrap in bubblewrap | |
| − | + | * Go to http://www.dna.iastate.edu/users/index.html | |
| − | + | * Login = eckdahl, Password = toad | |
| − | + | * Input number of reactions, choose Plasmid Template Type, hit Continue | |
| − | + | * Input names of template exactly as written on tubes | |
| − | + | * Input concentration in ug/ul (eg. 50 ng/ul = .050 ug/ul) | |
| − | + | * Input approximate insert size and approximate vector size | |
| − | + | * Input G00100 for Forward primer or G00101 for Reverse primer | |
| − | + | * Input “Eckdahl primer box” under Comments | |
| − | + | * Press Continue | |
| − | + | * Address Error Message if you can | |
| − | + | * Press Submit | |
| − | + | * Print page with Samples Submitted and Tracking Number | |
| − | + | * Place page and 50 tube(s) with DNA templates in FedEx box for shipping to | |
| − | + | DNA Sequencing Facility, Iowa State University, 1184 Molecular Biology Bldg, Ames, IA, 50011-3260 | |
| − | + | * Get data from http://dna.biotech.iastate.edu/users/dna_gid.html | |
Latest revision as of 17:03, 8 September 2010
- Prepare minipreps of DNA to be sequenced – concentration should be at least 50 ng/ul
- Put 10 ul of each DNA template in a 1.5 ml tube
- Label the DNA template tubes with a name not more than 8 characters
- Seal tubes with parafilm and place in 50 ml tube
- Wrap in bubblewrap
- Go to http://www.dna.iastate.edu/users/index.html
- Login = eckdahl, Password = toad
- Input number of reactions, choose Plasmid Template Type, hit Continue
- Input names of template exactly as written on tubes
- Input concentration in ug/ul (eg. 50 ng/ul = .050 ug/ul)
- Input approximate insert size and approximate vector size
- Input G00100 for Forward primer or G00101 for Reverse primer
- Input “Eckdahl primer box” under Comments
- Press Continue
- Address Error Message if you can
- Press Submit
- Print page with Samples Submitted and Tracking Number
- Place page and 50 tube(s) with DNA templates in FedEx box for shipping to
DNA Sequencing Facility, Iowa State University, 1184 Molecular Biology Bldg, Ames, IA, 50011-3260
- Get data from http://dna.biotech.iastate.edu/users/dna_gid.html
