Difference between revisions of "New Chaperone PCR"
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== Programed PCR Protocol == | == Programed PCR Protocol == | ||
− | Stage 1: | + | '''Stage 1:''' |
+ | |||
94ºC 10 minutes | 94ºC 10 minutes | ||
− | Stage 2: 20 cycles | + | '''Stage 2:''' |
+ | |||
+ | 20 cycles | ||
+ | |||
94ºC 15 seconds | 94ºC 15 seconds | ||
+ | |||
51ºC 15 seconds | 51ºC 15 seconds | ||
+ | |||
74ºC 2 minutes | 74ºC 2 minutes | ||
− | Stage 3: | + | '''Stage 3:''' |
+ | |||
74ºC 5 minutes | 74ºC 5 minutes |
Latest revision as of 19:10, 28 May 2014
Preparation
1. 2uL Bacteria
2. 0.5uL New Chaperone Mix
3. 7.5uL dH20
4. 10uL GoTac Green
Programed PCR Protocol
Stage 1:
94ºC 10 minutes
Stage 2:
20 cycles
94ºC 15 seconds
51ºC 15 seconds
74ºC 2 minutes
Stage 3:
74ºC 5 minutes