New Chaperone PCR

Preparation

1. 2uL Bacteria

2. 0.5uL New Chaperone Mix

3. 7.5uL dH20

4. 10uL GoTac Green

Programed PCR Protocol

Stage 1:

94ºC 10 minutes

Stage 2:

20 cycles

94ºC 15 seconds

51ºC 15 seconds

74ºC 2 minutes

Stage 3:

74ºC 5 minutes