Difference between revisions of "Status Of Building Parts"

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MWSU + Davidson Status of Building Sender and Receiver
 
MWSU + Davidson Status of Building Sender and Receiver
 +
 +
[http://partsregistry.org/AHL '''List of auto-inducers and their Sigma catalog numbers'''.]
  
 
plux-lac hybrid promoter:
 
plux-lac hybrid promoter:
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!width="65"|Size of Insert (w/ BioBrick ends)
 
!width="65"|Size of Insert (w/ BioBrick ends)
 
!width="150"|Verification using Gel Electrophoresis  
 
!width="150"|Verification using Gel Electrophoresis  
 +
!width="45"|Glycerol Stock
 
|-
 
|-
−
|BBa_F2620|| Accepts 3OC6HSL as an input and outputs PoPS||1102 bp||Appears to be reversed with J04430
+
|BBa_F2620|| Accepts 3OC6HSL as an input and outputs PoPS||1102 bp||Appears to be reversed with J04430|| Yes
 
|-
 
|-
−
|BBa_J04430|| GFP generator switched on by IPTG||1124 bp|| Appears to be reversed with F2620
+
|BBa_J04430|| GFP generator switched on by IPTG||1124 bp|| Appears to be reversed with F2620|| Yes
 
|-
 
|-
−
|BBa_P0440|| Produces tetR||881 bp|| Verified
+
|BBa_P0440|| Produces tetR||881 bp|| Verified|| Yes
 
|-
 
|-
−
|BBa_E0240|| GFP generator|| 917 bp||Verified
+
|BBa_E0240|| GFP generator|| 917 bp||Verified|| Yes
 
|-
 
|-
−
|BBa_I132623|| Lux receiver that produces luxR and outputs EYFP|| 2085 bp|| Much smaller on gel
+
|BBa_I132623|| Lux receiver that produces luxR and outputs EYFP|| 2085 bp|| Much smaller on gel ||Yes
 
|-
 
|-
−
|BBa_I0426|| Produces lasR and outputs ECFP followed by a lasR-PAI regulated promoter that outputs EYFP ||2839 bp|| Verified
+
|BBa_I0426|| Produces lasR and outputs ECFP followed by a lasR-PAI regulated promoter that outputs EYFP ||2839 bp|| Verified|| Yes
 
|-
 
|-
−
|BBa_C0061|| Produces luxI (w/ LVA tag) ||659 bp|| Verified
+
|BBa_C0061|| Produces luxI (w/ LVA tag) ||659 bp|| Verified|| Yes
 
|-
 
|-
 
|BBa_C0261|| Produces luxI (w/ RBS)|| 702 bp|| No successful Mini-Preps
 
|BBa_C0261|| Produces luxI (w/ RBS)|| 702 bp|| No successful Mini-Preps
 
|-
 
|-
−
|BBa_R0062|| luxR and HSL regulated promoter|| 96 bp|| Verified
+
|BBa_R0062|| luxR and HSL regulated promoter|| 96 bp|| Verified|| Yes
 
|-
 
|-
−
|BBa_I0466|| RhlR protein generator|| 983 bp|| Verified
+
|BBa_I0466|| RhlR protein generator|| 983 bp|| Verified|| Yes
 
|-
 
|-
−
|BBa_I0407|| Produces lasI and outputs ECFP|| 1674 bp|| Verified
+
|BBa_I0407|| Produces lasI and outputs ECFP|| 1674 bp|| Verified|| Yes
 
|-
 
|-
−
|BBa_R0079|| LasR and PAI regulated promoter || 198 bp|| Verified
+
|BBa_R0079|| LasR and PAI regulated promoter || 198 bp|| Verified|| Yes
 
|-
 
|-
 
|BBa_R0063|| LuxR and HSL regulated promoter|| 192 bp|| No successful Mini-Preps
 
|BBa_R0063|| LuxR and HSL regulated promoter|| 192 bp|| No successful Mini-Preps
 
|-
 
|-
−
|BBa_K09100|| Receiver for AHL and outputs GFP|| 1987 bp|| Verified
+
|BBa_K09100|| Receiver for AHL and outputs GFP|| 1987 bp|| Verified|| Yes
 
|-
 
|-
 
|BBa_J04450|| RFP coding device switched on by IPTG|| 2110 bp|| Much smaller on gel
 
|BBa_J04450|| RFP coding device switched on by IPTG|| 2110 bp|| Much smaller on gel
 
|-
 
|-
 +
|BBa_C0079|| lasR activator|| 797|| Verified|| Yes
 +
|-
 +
|BBa_S03156|| Ribosomal binding site followed by lasr coding region|| 840|| Verified|| Yes
 +
|-
 +
|BBa_S03154|| Ribosomal binding site followed by lasI coding region|| 726||
 +
|-
 +
|BBa_T9002||Inputs C06HSL and outputs GFP|| 1986||
 
|}  
 
|}  
 
-We will be sending all of these parts to be sequenced <br>
 
-We will be sending all of these parts to be sequenced <br>
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|-
 
|-
 
|} <br>
 
|} <br>
−
'''Parts we have Glycerol Stocks for'''
 
−
{| border="1" cellpadding="2"
 
−
!width="50"|Part
 
−
!width="225"|Description
 
−
|-
 
−
|R0062|| LuxR and HSL regulated promoter
 
−
|-
 
−
|R0063|| LuxR and HSL regulate promoter
 
−
|-
 
−
|P0440|| Produces tetR
 
−
|-
 
−
|I13263|| Lux Receiver
 
−
|-
 
−
|I0407|| Produces lasI and outputs ECFP
 
−
|-
 
−
|I0466||RhlR protein generator
 
−
|-
 
−
|K09100|| AHL receiver and outputs GFP
 
−
|-
 
−
|J04430|| GFP generator switched on by IPTG
 
−
|-
 
−
|E0240||GFP generator
 
−
|-
 
−
|F2620|| Accepts 30C6HSL as an input and outputs PoPS
 
−
|-
 
−
|J04450|| RFP coding device switched on by IPTG
 
−
|-
 
−
|C0061|| Produces luxI (w/ LVA tag
 
−
|-
 
−
|I0426|| Produces lasR and outputs ECFP followed by a lasR-PAI regulated promoter that outputs EYFP
 
−
|-
 
−
|R0079|| LasR and PAI regulated promoter
 
−
|-
 
−
|B0015|| Double terminator
 
−
|-
 
−
|R0011|| lacI regulated promoter
 
−
|-
 
−
|I13042|| lacIQ promoter
 
−
|-
 
−
|C0261||Produces luxI (w/ RBS)
 
−
|-
 
−
|MG1655|| E. Coli Genomic DNA
 
−
|-
 
−
|S03154||Ribosomal binding site followed by lasI coding region
 
−
|-
 
−
|S03156||Ribosomal binding site followed by lasR coding region
 
−
|-
 
−
|C0079|| lasR activator
 
−
|-
 
−
|}
 
 
[http://parts.mit.edu/igem07/index.php/USTC '''Look at USTC 2007 for logic gates''']
 
[http://parts.mit.edu/igem07/index.php/USTC '''Look at USTC 2007 for logic gates''']
  

Latest revision as of 14:14, 18 June 2008

MWSU + Davidson Status of Building Sender and Receiver

List of auto-inducers and their Sigma catalog numbers.

plux-lac hybrid promoter:

  • I751502
  • In the process of building another plux-lac hybrid promoter that is repressed by HSL and lacI

Responsible: Robert Cool, Erin Feeney and Pallavi Penumetcha

Parts we have Mini-Preps and Digestions for

Part Description Size of Insert (w/ BioBrick ends) Verification using Gel Electrophoresis Glycerol Stock
BBa_F2620 Accepts 3OC6HSL as an input and outputs PoPS 1102 bp Appears to be reversed with J04430 Yes
BBa_J04430 GFP generator switched on by IPTG 1124 bp Appears to be reversed with F2620 Yes
BBa_P0440 Produces tetR 881 bp Verified Yes
BBa_E0240 GFP generator 917 bp Verified Yes
BBa_I132623 Lux receiver that produces luxR and outputs EYFP 2085 bp Much smaller on gel Yes
BBa_I0426 Produces lasR and outputs ECFP followed by a lasR-PAI regulated promoter that outputs EYFP 2839 bp Verified Yes
BBa_C0061 Produces luxI (w/ LVA tag) 659 bp Verified Yes
BBa_C0261 Produces luxI (w/ RBS) 702 bp No successful Mini-Preps
BBa_R0062 luxR and HSL regulated promoter 96 bp Verified Yes
BBa_I0466 RhlR protein generator 983 bp Verified Yes
BBa_I0407 Produces lasI and outputs ECFP 1674 bp Verified Yes
BBa_R0079 LasR and PAI regulated promoter 198 bp Verified Yes
BBa_R0063 LuxR and HSL regulated promoter 192 bp No successful Mini-Preps
BBa_K09100 Receiver for AHL and outputs GFP 1987 bp Verified Yes
BBa_J04450 RFP coding device switched on by IPTG 2110 bp Much smaller on gel
BBa_C0079 lasR activator 797 Verified Yes
BBa_S03156 Ribosomal binding site followed by lasr coding region 840 Verified Yes
BBa_S03154 Ribosomal binding site followed by lasI coding region 726
BBa_T9002 Inputs C06HSL and outputs GFP 1986

-We will be sending all of these parts to be sequenced

Gels used to verify parts
Gel A
These are the parts run on a 0.5% gel.
GelA.jpg
Top portion of Gel A

Lane Part
1 MW
2 F0260
3 J04430
4 P0440
5 E0240
6 I13263
7 J0440

Bottom portion of Gel A

Lane Part
1 MW
2 C0061
3 I0466
4 I0407
5 I0426
6 K09100

GelB
These parts were run on a 2.5% gel.
GelB.jpg

Lane Part
1 MW
2 R0062
3 R0079
9 pTet-lac PCR product
10 MW

Look at USTC 2007 for logic gates

T9002 - no colonies
T9002.jpg

F2622 + E0240 (ligated/colonies coming up)
F2622.jpg + E0240.jpg

S03632 (ligations needed)
S03632.jpg
But BBa_S03632 is not available, so we will need to build our own version using the 3 parts below:
Parts: R0011 + C0261 + B0015
R0011b.jpg + C0261.jpg + B0015.jpg

R0011 + F1610 (minis, frag needed/colonies coming up, minis thurs)
R0011b.jpg + F1610.jpg

F2620 + E0240 (minis growing/ frag available)
F2620b.jpg + E0240.jpg

P0440
P0440.jpg

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