Status Of Building Parts
MWSU + Davidson Status of Building Sender and Receiver
List of auto-inducers and their Sigma catalog numbers.
plux-lac hybrid promoter:
- I751502
- In the process of building another plux-lac hybrid promoter that is repressed by HSL and lacI
Responsible: Robert Cool, Erin Feeney and Pallavi Penumetcha
Parts we have Mini-Preps and Digestions for
| Part | Description | Size of Insert (w/ BioBrick ends) | Verification using Gel Electrophoresis | Glycerol Stock |
|---|---|---|---|---|
| BBa_F2620 | Accepts 3OC6HSL as an input and outputs PoPS | 1102 bp | Appears to be reversed with J04430 | Yes |
| BBa_J04430 | GFP generator switched on by IPTG | 1124 bp | Appears to be reversed with F2620 | Yes |
| BBa_P0440 | Produces tetR | 881 bp | Verified | Yes |
| BBa_E0240 | GFP generator | 917 bp | Verified | Yes |
| BBa_I132623 | Lux receiver that produces luxR and outputs EYFP | 2085 bp | Much smaller on gel | Yes |
| BBa_I0426 | Produces lasR and outputs ECFP followed by a lasR-PAI regulated promoter that outputs EYFP | 2839 bp | Verified | Yes |
| BBa_C0061 | Produces luxI (w/ LVA tag) | 659 bp | Verified | Yes |
| BBa_C0261 | Produces luxI (w/ RBS) | 702 bp | No successful Mini-Preps | |
| BBa_R0062 | luxR and HSL regulated promoter | 96 bp | Verified | Yes |
| BBa_I0466 | RhlR protein generator | 983 bp | Verified | Yes |
| BBa_I0407 | Produces lasI and outputs ECFP | 1674 bp | Verified | Yes |
| BBa_R0079 | LasR and PAI regulated promoter | 198 bp | Verified | Yes |
| BBa_R0063 | LuxR and HSL regulated promoter | 192 bp | No successful Mini-Preps | |
| BBa_K09100 | Receiver for AHL and outputs GFP | 1987 bp | Verified | Yes |
| BBa_J04450 | RFP coding device switched on by IPTG | 2110 bp | Much smaller on gel | |
| BBa_C0079 | lasR activator | 797 | Verified | Yes |
| BBa_S03156 | Ribosomal binding site followed by lasr coding region | 840 | Verified | Yes |
| BBa_S03154 | Ribosomal binding site followed by lasI coding region | 726 | ||
| BBa_T9002 | Inputs C06HSL and outputs GFP | 1986 |
-We will be sending all of these parts to be sequenced
Gels used to verify parts
Gel A
These are the parts run on a 0.5% gel.
Top portion of Gel A
| Lane | Part |
|---|---|
| 1 | MW |
| 2 | F0260 |
| 3 | J04430 |
| 4 | P0440 |
| 5 | E0240 |
| 6 | I13263 |
| 7 | J0440 |
Bottom portion of Gel A
| Lane | Part |
|---|---|
| 1 | MW |
| 2 | C0061 |
| 3 | I0466 |
| 4 | I0407 |
| 5 | I0426 |
| 6 | K09100 |
GelB
These parts were run on a 2.5% gel.
| Lane | Part |
|---|---|
| 1 | MW |
| 2 | R0062 |
| 3 | R0079 |
| 9 | pTet-lac PCR product |
| 10 | MW |
Look at USTC 2007 for logic gates
T9002 - no colonies
F2622 + E0240 (ligated/colonies coming up)
+ 
S03632 (ligations needed)
But BBa_S03632 is not available, so we will need to build our own version using the 3 parts below:
Parts: R0011 + C0261 + B0015
+ 
+ 
R0011 + F1610 (minis, frag needed/colonies coming up, minis thurs)
+ 
F2620 + E0240 (minis growing/ frag available)
+
