Difference between revisions of "New Chaperone PCR"
From GcatWiki
| Line 18: | Line 18: | ||
Stage 2: 20 cycles | Stage 2: 20 cycles | ||
94ºC 15 seconds | 94ºC 15 seconds | ||
| + | |||
51ºC 15 seconds | 51ºC 15 seconds | ||
| + | |||
74ºC 2 minutes | 74ºC 2 minutes | ||
Stage 3: | Stage 3: | ||
74ºC 5 minutes | 74ºC 5 minutes | ||
Revision as of 19:08, 28 May 2014
Preparation
1. 2uL Bacteria
2. 0.5uL New Chaperone Mix
3. 7.5uL dH20
4. 10uL GoTac Green
Programed PCR Protocol
Stage 1: 94ºC 10 minutes
Stage 2: 20 cycles 94ºC 15 seconds
51ºC 15 seconds
74ºC 2 minutes
Stage 3: 74ºC 5 minutes
