Difference between revisions of "New Chaperone PCR"
From GcatWiki
| Line 13: | Line 13: | ||
== Programed PCR Protocol == | == Programed PCR Protocol == | ||
| − | Stage 1: | + | '''Stage 1:''' |
| + | |||
94ºC 10 minutes | 94ºC 10 minutes | ||
| − | Stage 2: | + | '''Stage 2:''' |
20 cycles | 20 cycles | ||
| Line 25: | Line 26: | ||
74ºC 2 minutes | 74ºC 2 minutes | ||
| + | ''' | ||
| + | Stage 3:''' | ||
| − | |||
74ºC 5 minutes | 74ºC 5 minutes | ||
Revision as of 19:09, 28 May 2014
Preparation
1. 2uL Bacteria
2. 0.5uL New Chaperone Mix
3. 7.5uL dH20
4. 10uL GoTac Green
Programed PCR Protocol
Stage 1:
94ºC 10 minutes
Stage 2:
20 cycles
94ºC 15 seconds
51ºC 15 seconds
74ºC 2 minutes Stage 3:
74ºC 5 minutes
