Difference between revisions of "MWSU protocols"
From GcatWiki
| Line 35: | Line 35: | ||
[[BioBrick Ligations]] | [[BioBrick Ligations]] | ||
| − | |||
| − | |||
| − | |||
| − | |||
[[Ligation and Transformation]] | [[Ligation and Transformation]] | ||
| Line 55: | Line 51: | ||
[[Measuring Fluorescence in Bacteria]] | [[Measuring Fluorescence in Bacteria]] | ||
| + | |||
| + | '''DNA and E coli''' | ||
| + | |||
| + | [[DNA glycerol stock]] | ||
Revision as of 19:35, 20 May 2013
Purification of DNA Isolation of Genomic DNA from Bacteria
PCR
Recombinant DNA Production
Golden Gate Assembly Protocol for BsmB1
BioBrick Digestions for Fragment and Vector Preparation
Direct Synthesis with Overlapping Oligos
Ethanol Precipitation of Vector DNA
Reducing Background from Double Digested Vector
Ligation and Transformation
Screening Clones
Diagnostic RP Digestion for Checking Insert Size
DNA Sequencing at Iowa State University
Measuring Phenotypes
Measuring Fluorescence in Bacteria
DNA and E coli
