Difference between revisions of "Biology"
From GcatWiki
Macampbell (talk | contribs) |
Macampbell (talk | contribs) |
||
Line 3: | Line 3: | ||
<u>High Priority = more people</u> | <u>High Priority = more people</u> | ||
− | * '''Phoebe and Claire:''' eCDM8 working to make theophylline with biosensor and fitness modules (Tet | + | * '''Phoebe and Claire:''' eCDM8 working to make theophylline with biosensor and fitness modules (Tet<sup>R</sup>) to report out. <br> |
Which plasmid carries which device?<br> | Which plasmid carries which device?<br> | ||
Biosensor<br> | Biosensor<br> |
Revision as of 14:47, 30 May 2013
Topics to Investigate
High Priority = more people
- Phoebe and Claire: eCDM8 working to make theophylline with biosensor and fitness modules (TetR) to report out.
Which plasmid carries which device?
Biosensor
Fitness (tetA)
feeds into stress module
- Sachii and Jess: Determine the junction sequences and associated primers
(use existing rules)
build scaffold for insert segments
include orI (collaborate with copy number research)
include drug resistance gene
test JGG design
- Spencer and Sara: test fitness module with adhE
compare with tetA
fine tune tetA resistance
combine with tetA
Does theophylline diffuse across the membrane and thus the system fails?
- Brandon and Erich: Copy number of plasmids
Can we alter Ori to produce a range of plasmid densities in E. coli?
Second Order Priority = one person
- produce different CDM alleles
- swap out different components (promoters, RBS, alleles)
- test out programmed evolution
- Stress module needs more work (see eCDM8 outputs)