Difference between revisions of "MWSU protocols"
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Revision as of 14:15, 10 March 2014
Purification of DNA Isolation of Genomic DNA from Bacteria
PCR
Template Preparation for RT-qPCR
Recombinant DNA Production
Zymo Research Plasmid Minipreps
Golden Gate Assembly Protocol for BsmB1
BioBrick Digestions for Fragment and Vector Preparation
Direct Synthesis with Overlapping Oligos
Ethanol Precipitation of Vector DNA
Reducing Background from Double Digested Vector
Ligation and Transformation
Electroporation Transformation
Screening Clones
Diagnostic RP Digestion for Checking Insert Size
DNA Sequencing at Iowa State University
Measuring Phenotypes
Measuring Fluorescence in Bacteria
DNA and E coli
