Difference between revisions of "New Chaperone PCR"
From GcatWiki
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94ºC 10 minutes | 94ºC 10 minutes | ||
| − | Stage 2: 20 cycles | + | Stage 2: |
| + | |||
| + | 20 cycles | ||
| + | |||
94ºC 15 seconds | 94ºC 15 seconds | ||
Revision as of 19:09, 28 May 2014
Preparation
1. 2uL Bacteria
2. 0.5uL New Chaperone Mix
3. 7.5uL dH20
4. 10uL GoTac Green
Programed PCR Protocol
Stage 1: 94ºC 10 minutes
Stage 2:
20 cycles
94ºC 15 seconds
51ºC 15 seconds
74ºC 2 minutes
Stage 3: 74ºC 5 minutes
