Difference between revisions of "Elise"
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.1g of organ used, we are not sure that it's the right part- could be connective tissue etc. | .1g of organ used, we are not sure that it's the right part- could be connective tissue etc. | ||
Anything in cDNA, by definition is transcribed mRNA, more stable as DNA | Anything in cDNA, by definition is transcribed mRNA, more stable as DNA | ||
| + | mRNA to cDNA using reverse transcriptase | ||
| + | Advantage to fragmenting RNA prior to cDNA synthesis-random fragmentation allows for a lot more reads, because I long strand of RNA would just have resulted in short reads from the two ends | ||
| + | Every possible hexamer is generated is used at end of primer in order for synthesis to occur | ||
Revision as of 19:52, 12 January 2016
THIS IS ELISE.
.1g of organ used, we are not sure that it's the right part- could be connective tissue etc. Anything in cDNA, by definition is transcribed mRNA, more stable as DNA mRNA to cDNA using reverse transcriptase Advantage to fragmenting RNA prior to cDNA synthesis-random fragmentation allows for a lot more reads, because I long strand of RNA would just have resulted in short reads from the two ends Every possible hexamer is generated is used at end of primer in order for synthesis to occur
