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.1g of organ used, we are not sure that it's the right part- could be connective tissue etc. Anything in cDNA, by definition is transcribed mRNA, more stable as DNA mRNA to cDNA using reverse transcriptase Advantage to fragmenting RNA prior to cDNA synthesis-random fragmentation allows for a lot more reads, because I long strand of RNA would just have resulted in short reads from the two ends Every possible hexamer is generated is used at end of primer in order for synthesis to occur

Research Our results indicate that 􏰀2,000 genes show significant changes in expression in the small intestine following feeding, includ- ing genes involved in intestinal morphology and function (e.g., hy- drolases, microvillus proteins, trafficking and transport proteins), as well as genes involved in cell division and apoptosis. Mass-specific rates of D-glucose, L-proline, and L-leucine uptake varied significantly among sampling times (P values 􏰈 0.0009), as each nutrient significantly increased uptake within 12–24 h of feeding (Fig. 5, D–F, respectively). (http://www.bio.davidson.edu/courses/Bio343/2016/4a_Todd%20Castoe%20Python%20RNAseq_2015.pdf)

Trying to access full article-related to SGLT1 gene and glucose uptake http://www.ncbi.nlm.nih.gov/pubmed/1616036

2/04/16 2/09/16 2/11/16 2/23/16 2/25/16 3/8/16