Difference between revisions of "Status Of Building Parts"
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!width="150"|Verification using PCR | !width="150"|Verification using PCR | ||
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− | |BBa_F2620|| Accepts 3OC6HSL as an input and outputs PoPS||1102||Verified | + | |BBa_F2620|| Accepts 3OC6HSL as an input and outputs PoPS||1102 bp||Appears to be reversed with J04430 |
+ | |- | ||
+ | |BBa_J04430|| GFP generator switched on by IPTG||1124 bp|| Appears to be reversed with F2620 | ||
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+ | |BBa_P0440|| Produces tetR||881 bp|| Verified | ||
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Revision as of 18:28, 5 June 2008
MWSU + Davidson Status of Building Sender and Receiver
plux-lac hybrid promoter:
- I751502
- In the process of building another plux-lac hybrid promoter that is repressed by HSL and lacI
Responsible: Robert Cool, Erin Feeney and Pallavi Penumetcha
Parts we have Mini-Preps and Digestions for
Part | Description | Size of Insert (w/ BioBrick ends) | Verification using PCR |
---|---|---|---|
BBa_F2620 | Accepts 3OC6HSL as an input and outputs PoPS | 1102 bp | Appears to be reversed with J04430 |
BBa_J04430 | GFP generator switched on by IPTG | 1124 bp | Appears to be reversed with F2620 |
BBa_P0440 | Produces tetR | 881 bp | Verified |
Look at USTC 2007 for logic gates
T9002 - no colonies
F2622 + E0240 (ligated/colonies coming up)
+
S03632 (ligations needed)
But BBa_S03632 is not available, so we will need to build our own version using the 3 parts below:
Parts: R0011 + C0261 + B0015
+
+
R0011 + F1610 (minis, frag needed/colonies coming up, minis thurs)
+
F2620 + E0240 (minis growing/ frag available)
+