DNA Sequencing at Iowa State University

DNA Sequencing at Iowa State University 1. Prepare minipreps of DNA to be sequenced – concentration should be at least 50 ng/ul 2. Put 10 ul of each DNA template in a 1.5 ml tube 3. Label the DNA template tubes with a name not more than 8 characters 4. Seal tubes with parafilm and place in 50 ml tube 5. Wrap in bubblewrap 6. Go to http://www.dna.iastate.edu/users/index.html a. Login = eckdahl b. Password = toad 7. Input number of reactions, choose Plasmid Template Type, hit Continue 8. Input names of template exactly as written on tubes 9. Input concentration in ug/ul (eg. 50 ng/ul = .050 ug/ul) 10. Input approximate insert size and approximate vector size 11. Input G00100 for Forward primer or G00101 for Reverse primer 12. Input “Eckdahl primer box” under Comments 13. Press Continue 14. Address Error Message if you can 15. Press Submit 16. Print page with Samples Submitted and Tracking Number 17. Place page and 50 tube(s) with DNA templates in FedEx box for shipping