New Chaperone PCR

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Preparation

1. 2uL Bacteria

2. 0.5uL New Chaperone Mix

3. 7.5uL dH20

4. 10uL GoTac Green

Programed PCR Protocol

Stage 1: 94ºC 10 minutes

Stage 2: 20 cycles 94ºC 15 seconds

51ºC 15 seconds

74ºC 2 minutes

Stage 3: 74ºC 5 minutes

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