New Chaperone PCR
From GcatWiki
Preparation
1. 2uL Bacteria
2. 0.5uL New Chaperone Mix
3. 7.5uL dH20
4. 10uL GoTac Green
Programed PCR Protocol
Stage 1:
94ºC 10 minutes
Stage 2:
20 cycles
94ºC 15 seconds
51ºC 15 seconds
74ºC 2 minutes
Stage 3:
74ºC 5 minutes