Difference between revisions of "Biology"
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fine tune tetA resistance<br> | fine tune tetA resistance<br> | ||
combine with tetA<br> | combine with tetA<br> | ||
| − | Does theophylline diffuse across the membrane and thus the system fails<br> | + | Does theophylline diffuse across the membrane and thus the system fails?<br> |
* Copy number of plasmids <br> | * Copy number of plasmids <br> | ||
Can we alter Ori to produce a range of plasmid densities in ''E. coli''? <br> | Can we alter Ori to produce a range of plasmid densities in ''E. coli''? <br> | ||
Revision as of 14:27, 30 May 2013
Topics to Investigate
High Priority = more people
- eCDM8 working to make theophylline with biosensor and fitness modules (Tet^R) to report out.
Which plasmid carries which device?
- Determine the junction sequences and associated primers
(use existing rules)
build scaffold for insert segments
include orI (collaborate with copy number research)
include drug resistance gene
test JGG design
- test fitness module with adhE
compare with tetA
fine tune tetA resistance
combine with tetA
Does theophylline diffuse across the membrane and thus the system fails?
- Copy number of plasmids
Can we alter Ori to produce a range of plasmid densities in E. coli?
Second Order Priority = one person
- produce different CDM alleles
- swap out different components (promoters, RBS, alleles)
- test out programmed evolution
- Stress module needs more work (see eCDM8 outputs)
