Davidson Missouri W/MWSU protocols

What to do with a new clone

Diagnostic RP Digestion for Checking Insert Size

DNA Sequencing at Iowa State University

BioBrick Digestions for Fragment and Vector Preparation

Reducing Background from Double Digested Vector

Pouring an Agarose Gel

Fragment Purification

Ethanol Precipitation of Vector DNA

BioBrick Ligations

Ligation and Transformation

Colony PCR

Standard PCR

Isolation of Genomic DNA from Bacteria

Resuspending Oligos

Direct Synthesis with Overlapping Oligos

Bacterial Media

Measuring Fluorescence in Bacteria