Difference between revisions of "MWSU protocols"
From GcatWiki
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[[Resuspending Oligos]] | [[Resuspending Oligos]] | ||
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'''Recombinant DNA Production''' | '''Recombinant DNA Production''' | ||
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[[Diagnostic RP Digestion for Checking Insert Size]] | [[Diagnostic RP Digestion for Checking Insert Size]] | ||
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[[DNA Sequencing at Iowa State University]] | [[DNA Sequencing at Iowa State University]] | ||
Revision as of 14:34, 21 January 2012
Purification of DNA
Isolation of Genomic DNA from Bacteria
PCR
Recombinant DNA Production
BioBrick Digestions for Fragment and Vector Preparation
Direct Synthesis with Overlapping Oligos
Ethanol Precipitation of Vector DNA
Reducing Background from Double Digested Vector
Ligation and Transformation
Screening Clones
Diagnostic RP Digestion for Checking Insert Size
DNA Sequencing at Iowa State University
Measuring Phenotypes
