Difference between revisions of "MWSU protocols"
Macampbell (talk | contribs) (Created page with 'What to do with a new clone Diagnostic RP Digestion for Checking Insert Size DNA Sequencing at Iowa State University [[BioBrick Digestions for Fragment and Vector …') |
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| − | [[ | + | '''Purification of DNA''' |
| + | |||
| + | [[Isolation of Genomic DNA from Bacteria]] | ||
| + | |||
| + | [[Gel Purification New England BioLabs Monarch]] | ||
| + | |||
| + | '''PCR''' | ||
| + | |||
| + | [[iPCR]] | ||
| + | |||
| + | [[Gradient/Standard PCR]] | ||
| + | |||
| + | [[Resuspending Oligos]] | ||
| + | |||
| + | [[Davidson_Missouri_W/colony_PCR | Colony PCR]] | ||
| + | |||
| + | [[Template Preparation for RT-qPCR]] | ||
| + | |||
| + | [[New Chaperone PCR]] | ||
| + | |||
| + | [[LongAmp PCR]] | ||
| + | |||
| + | '''Recombinant DNA Production''' | ||
| − | [[ | + | [[Zymo Research Plasmid Minipreps]] |
| − | [[DNA | + | [[Zymo Research Clean and Concentrate for Plasmid DNA]] |
| − | [[ | + | [[Golden Gate Assembly Protocol]] |
| − | [[ | + | [[Golden Gate Assembly Single Molecule Protocol]] |
[[Pouring an Agarose Gel]] | [[Pouring an Agarose Gel]] | ||
| + | |||
| + | [[BioBrick Digestions for Fragment and Vector Preparation]] | ||
[[Fragment Purification]] | [[Fragment Purification]] | ||
| + | |||
| + | [[Gibson Assembly]] | ||
| + | |||
| + | [[Direct Synthesis with Overlapping Oligos]] | ||
| + | |||
| + | [[Annealing Oligos for Cloning]] | ||
[[Ethanol Precipitation of Vector DNA]] | [[Ethanol Precipitation of Vector DNA]] | ||
| + | |||
| + | [[Reducing Background from Double Digested Vector]] | ||
| + | |||
| + | [[File: PClone_Procedure_for_GCAT_SB_Workshop_2014_new_version.pptx]] | ||
| + | |||
| + | '''Ligation and Transformation''' | ||
[[BioBrick Ligations]] | [[BioBrick Ligations]] | ||
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[[Ligation and Transformation]] | [[Ligation and Transformation]] | ||
| − | [[ | + | [[Electroporation Transformation]] |
| + | |||
| + | [[SOC Protocol for Transformations]] | ||
| + | |||
| + | '''Screening Clones''' | ||
| + | |||
| + | [[Diagnostic RP Digestion for Checking Insert Size]] | ||
| + | |||
| + | [[DNA Sequencing at Iowa State University]] | ||
| + | |||
| + | [[DNA Sequencing at Eurofins]] | ||
| + | |||
| + | [[What to do with a new clone]] | ||
| + | |||
| + | [[How to make a new Registry page]] | ||
| + | |||
| + | '''Measuring Phenotypes''' | ||
| + | |||
| + | [[Measuring Fluorescence in Bacteria]] | ||
| + | |||
| + | [[Camera Settings for Taking Pictures of Plates]] | ||
| − | + | '''DNA and E coli''' | |
| − | [[ | + | [[GCAT Library of Quality Parts]] |
| − | [[ | + | [[MWSU Freezer Parts]] |
| − | + | '''Working With Bacteria''' | |
[[Bacterial Media]] | [[Bacterial Media]] | ||
| − | [[ | + | [[Washing Beads]] |
| + | |||
| + | [[Cleaning Plate Replication Pads]] | ||
Latest revision as of 20:08, 14 June 2018
Purification of DNA
Isolation of Genomic DNA from Bacteria
Gel Purification New England BioLabs Monarch
PCR
Template Preparation for RT-qPCR
Recombinant DNA Production
Zymo Research Plasmid Minipreps
Zymo Research Clean and Concentrate for Plasmid DNA
Golden Gate Assembly Single Molecule Protocol
BioBrick Digestions for Fragment and Vector Preparation
Direct Synthesis with Overlapping Oligos
Ethanol Precipitation of Vector DNA
Reducing Background from Double Digested Vector
File:PClone Procedure for GCAT SB Workshop 2014 new version.pptx
Ligation and Transformation
Electroporation Transformation
SOC Protocol for Transformations
Screening Clones
Diagnostic RP Digestion for Checking Insert Size
DNA Sequencing at Iowa State University
How to make a new Registry page
Measuring Phenotypes
Measuring Fluorescence in Bacteria
Camera Settings for Taking Pictures of Plates
DNA and E coli
Working With Bacteria
