Difference between revisions of "Davidson Missouri W/Davidson Protocols"

Revision as of 17:38, 30 June 2009

1. How to Keep a Lab Notebook
2. Common molecular reagents
3. Standard Assembly
4. BioBrick Ends
5. Compatibility of Plasmids
6. Building dsDNA with Oligos
7. Setting up PCR mixtures
8. PCR and Mg²⁺ concentration
9. Clean and Concentrate DNA (after PCR, before digestion)
10. Pouring an agarose gel
11. Calculate MWs
12. Digest DNA with restriction enzymes
13. Double Digest Guide
14. Ethanol Precipitate DNA (short protocol)
15. 1kb MW markers
16. Shrimp Alkaline Phosphatase
17. Qiagen QIAquick Gel Purification
18. Qiagen QIAquick Column Regeneration Protocol
19. ElectroElute Gel Purification
20. Ligation Protocol
21. Heat Shock Transformation OR Short version of Heat Shock
22. Zippy Transformation
23. TSS Competent Cell Transformation
24. Colony PCR to Screen for Successful Ligations
25. Promega miniprep
26. Choices for Transformation: Heat Shock vs. Zyppy
27. Choices for Mini-Preps: Promega vs. Zyppy
28. Making dsDNA Using Primer Dimers
29. Genomic Insertion Protocol
30. glycerolstocks How to Make Glycerol Stocks of Bacteria
31. When inducing with IPTG, use 3 µL of stock (0.2 g/mL = 20% w/v) to every 1 mL of LB or other liquid.
32. When inducing with Arabinose, use "2 µL of stock" (10% w/v L-Arabinose) "to every 1 mL" of LB or other liquid.

Computer Tools We Use

1. All Sites In One Place
2. Optimize your Gel
3. Gene Splitting Web Site
4. PCR Primers w/ BioBricks
5. Promega T_m Calculator
6. List of auto-inducers and their catalog numbers
7. Sequencing at CUGI
8. Analyzing Sequences with aPe
9. Lance-olator Oligos for dsDNA assembly
10. Access the GCAT-alog of Davidson and MWSU DNA Freezer Stocks