Difference between revisions of "Davidson Missouri W/Davidson Protocols"
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#[http://www.bio.davidson.edu/courses/Molbio/Protocols/MiniPrep_list.html Choices for Mini-Preps: Promega vs. Zyppy] | #[http://www.bio.davidson.edu/courses/Molbio/Protocols/MiniPrep_list.html Choices for Mini-Preps: Promega vs. Zyppy] | ||
# [[Davidson Missouri W/Primer_dimer| Making dsDNA Using Primer Dimers]] | # [[Davidson Missouri W/Primer_dimer| Making dsDNA Using Primer Dimers]] | ||
| + | # [[glycerolstocks How to Make Glycerol Stocks of Bacteria]] | ||
# When inducing with IPTG, use '''3 µL of stock''' (0.2 µg/mL = 20% w/v) '''to every 1 mL''' of LB or other liquid. | # When inducing with IPTG, use '''3 µL of stock''' (0.2 µg/mL = 20% w/v) '''to every 1 mL''' of LB or other liquid. | ||
# When inducing with Arabinose, use "2 µL of stock" (10% w/v L-Arabinose) "to every 1 mL" of LB or other liquid. | # When inducing with Arabinose, use "2 µL of stock" (10% w/v L-Arabinose) "to every 1 mL" of LB or other liquid. | ||
Revision as of 14:32, 5 February 2009
- How to Keep a Lab Notebook
- Common molecular reagents
- Standard Assembly
- BioBrick Ends
- Compatibility of Plasmids
- Building dsDNA with Oligos
- Setting up PCR mixtures
- PCR and Mg2+ concentration
- Clean and Concentrate DNA (after PCR, before digestion)
- Pouring an agarose gel
- Calculate MWs
- Digest DNA with restriction enzymes
- Double Digest Guide
- Ethanol Precipitate DNA (short protocol)
- 1kb MW markers
- Shrimp Alkaline Phosphatase
- Qiagen QIAquick Gel Purification
- Qiagen QIAquick Column Regeneration Protocol
- ElectroElute Gel Purification
- Ligation Protocol
- Heat Shock Transformation OR Short version of Heat Shock
- Zippy Transformation
- Colony PCR to Screen for Successful Ligations
- Promega miniprep
- Choices for Transformation: Heat Shock vs. Zyppy
- Choices for Mini-Preps: Promega vs. Zyppy
- Making dsDNA Using Primer Dimers
- glycerolstocks How to Make Glycerol Stocks of Bacteria
- When inducing with IPTG, use 3 µL of stock (0.2 µg/mL = 20% w/v) to every 1 mL of LB or other liquid.
- When inducing with Arabinose, use "2 µL of stock" (10% w/v L-Arabinose) "to every 1 mL" of LB or other liquid.
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