Difference between revisions of "Davidson Missouri W/Davidson Protocols"
From GcatWiki
Wideloache (talk | contribs) |
Wideloache (talk | contribs) |
||
| Line 21: | Line 21: | ||
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/Promegacompcells.pdf Heat Shock Transformation] OR [http://www.bio.davidson.edu/courses/Molbio/Protocols/transformation.html Short version of Heat Shock] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/Promegacompcells.pdf Heat Shock Transformation] OR [http://www.bio.davidson.edu/courses/Molbio/Protocols/transformation.html Short version of Heat Shock] | ||
#[http://www.bio.davidson.edu/courses/Molbio/Protocols/Zippy_Transformation.html Zippy Transformation] | #[http://www.bio.davidson.edu/courses/Molbio/Protocols/Zippy_Transformation.html Zippy Transformation] | ||
| − | #[[TSS Competent Cells]] | + | #[[TSS Competent Cells| TSS Competent Cell Transformation]] |
#[http://www.bio.davidson.edu/courses/Molbio/Protocols/ColonyPCR_Screening.html Colony PCR to Screen for Successful Ligations] | #[http://www.bio.davidson.edu/courses/Molbio/Protocols/ColonyPCR_Screening.html Colony PCR to Screen for Successful Ligations] | ||
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/miniprepPrmega.html Promega miniprep] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/miniprepPrmega.html Promega miniprep] | ||
Revision as of 19:44, 30 April 2009
- How to Keep a Lab Notebook
- Common molecular reagents
- Standard Assembly
- BioBrick Ends
- Compatibility of Plasmids
- Building dsDNA with Oligos
- Setting up PCR mixtures
- PCR and Mg2+ concentration
- Clean and Concentrate DNA (after PCR, before digestion)
- Pouring an agarose gel
- Calculate MWs
- Digest DNA with restriction enzymes
- Double Digest Guide
- Ethanol Precipitate DNA (short protocol)
- 1kb MW markers
- Shrimp Alkaline Phosphatase
- Qiagen QIAquick Gel Purification
- Qiagen QIAquick Column Regeneration Protocol
- ElectroElute Gel Purification
- Ligation Protocol
- Heat Shock Transformation OR Short version of Heat Shock
- Zippy Transformation
- TSS Competent Cell Transformation
- Colony PCR to Screen for Successful Ligations
- Promega miniprep
- Choices for Transformation: Heat Shock vs. Zyppy
- Choices for Mini-Preps: Promega vs. Zyppy
- Making dsDNA Using Primer Dimers
- glycerolstocks How to Make Glycerol Stocks of Bacteria
- When inducing with IPTG, use 3 µL of stock (0.2 g/mL = 20% w/v) to every 1 mL of LB or other liquid.
- When inducing with Arabinose, use "2 µL of stock" (10% w/v L-Arabinose) "to every 1 mL" of LB or other liquid.
Web Tools We Use
