Difference between revisions of "Davidson Missouri W/Davidson Protocols"

Revision as of 16:13, 18 November 2009

1. How to Keep a Lab Notebook
2. Common molecular reagents
3. Using degradation tags on proteins such as GFP
4. Standard Assembly
5. BioBrick Ends
6. Compatibility of Plasmids
7. Building dsDNA with Oligos
8. Setting up PCR mixtures
9. PCR and Mg²⁺ concentration
10. Clean and Concentrate DNA (after PCR, before digestion)
11. Pouring an agarose gel
12. Calculate MWs
13. Digest DNA with restriction enzymes
14. Double Digest Guide
15. Ethanol Precipitate DNA (short protocol)
16. 1kb MW markers
17. Shrimp Alkaline Phosphatase
18. Qiagen QIAquick Gel Purification
19. Qiagen QIAquick Column Regeneration Protocol
20. ElectroElute Gel Purification
21. Ligation Protocol
22. Heat Shock Transformation OR Short version of Heat Shock
23. Zippy Transformation
24. TSS Competent Cell Transformation
25. Colony PCR to Screen for Successful Ligations
26. Promega miniprep
27. Choices for Transformation: Heat Shock vs. Zyppy
28. Choices for Mini-Preps: Promega vs. Zyppy
29. Making dsDNA Using Primer Dimers
30. Genomic Insertion Protocol
31. glycerolstocks How to Make Glycerol Stocks of Bacteria
32. When inducing with IPTG, use 3 µL of stock (0.2 g/mL = 20% w/v) to every 1 mL of LB or other liquid.
33. When inducing with Arabinose, use "2 µL of stock" (10% w/v L-Arabinose) "to every 1 mL" of LB or other liquid.

Computer Tools We Use

1. All Sites In One Place
2. Optimize your Gel
3. Gene Splitting Web Site
4. PCR Primers w/ BioBricks
5. Promega T_m Calculator
6. List of auto-inducers and their catalog numbers
7. Sequencing at Agencourt Bioscience
8. Sequencing at CUGI
9. Analyzing Sequences with aPe
10. Lance-olator Oligos for dsDNA assembly
11. Access the GCAT-alog of Davidson and MWSU DNA Freezer Stocks
12. Using Apes (A Plasmid Editor)